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  PIAS Proteins as Repressors of Oct4 Function

Tolkunova, E., Malashicheva, A., Parfenov, V. N., Sustmann, C., Grosschedl, R., & Tomilin, A. (2007). PIAS Proteins as Repressors of Oct4 Function. Journal of Molecular Biology (London), 374, 1200-1212. doi:10.1016/j.jmb.2007.09.081.

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 Creators:
Tolkunova, Elena1, Author              
Malashicheva, Anna1, Author              
Parfenov, Vladimir N.2, Author
Sustmann, Claudio3, Author              
Grosschedl, Rudolf3, Author              
Tomilin, Alexey1, Author              
Affiliations:
1Department of Developmental Biology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243650              
2External Organizations, ou_persistent22              
3Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              

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Free keywords: PIAS; POU; Oct4; SUMO; transcriptional repression
 Abstract: The POU domain transcription factor Oct4 plays essential functions in the maintenance of pluripotent embryonic and germ cells of mammals. Molecular mechanisms of Oct4 action remain poorly understood. To isolate modulators of Oct4 activity, we performed a yeast two-hybrid screen with the Oct4 POU domain as a bait and isolated PIASy as an Oct4-interacting protein. Oct4 and PIASy interact in vivo via their POU domain and SAP-domain-containing N terminus, respectively. PIASy does not enhance Oct4 sumoylation but acts as a potent inhibitor of Oct4-mediated transcriptional activation, sequestering Oct4 protein from the vicinity of Cajal bodies and splicing speckles to the nuclear periphery. These modes of PIASy action are uncoupled from its sumoylation activity. Other PIAS family members, PIAS1 and PIAS3, can also interact with Oct4 in vivo and target Oct4 to the nuclear periphery, depending on cellular context. We propose that Oct4 inhibition, mediated by this new class of transcriptional partners, might be instrumental during mammalian development.

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Language(s): eng - English
 Dates: 2007-12-14
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.jmb.2007.09.081
 Degree: -

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Title: Journal of Molecular Biology (London)
  Other : J Mol Biol
Source Genre: Journal
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Publ. Info: London : Academic Press
Pages: - Volume / Issue: 374 Sequence Number: - Start / End Page: 1200 - 1212 Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/954922646042