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  Testing gene function early in the B cell lineage in mb 1-cre mice

Hobeika, E., Thiemann, S., Storch, B., Jumaa, H., Nielsen, P., Pelanda, R., et al. (2006). Testing gene function early in the B cell lineage in mb 1-cre mice. Proceedings of the National Academy of Sciences of the United States of America, 103, 13789-13794.

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 Creators:
Hobeika, E.1, Author           
Thiemann, S., Author
Storch, B.1, Author           
Jumaa, H.1, Author           
Nielsen, P.J.1, Author           
Pelanda, R.1, Author           
Reth, M.1, Author           
Affiliations:
1Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645              

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Free keywords: Dnmt1; SRp20; loxP; enhanced yellow fluorescent protein; lymphocyte
 Abstract: The mb1 gene encodes the Ig-α signaling subunit of the B cell antigen receptor and is expressed exclusively in B cells beginning at the very early pro-B cell stage in the bone marrow. We examine here the efficacy of the mb1 gene as a host locus for cre recombinase expression in B cells. We show that by integrating a humanized cre recombinase into the mb1 locus we obtain extraordinarily efficient recombination of loxP sites in the B cell lineage. The results from a variety of reporter genes including the splicing factor SRp20 and the DNA methylase Dnmt1 suggest that mb1-cre is probably the best model so far described for pan-B cell-specific cre expression. The availablity of a mouse line with efficient cre-mediated recombination at an early developmental stage in the B lineage provides an opportunity to study the role of various genes specifically in B cell development and function.

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Language(s): eng - English
 Dates: 2006-09-12
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 306577
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Alternative Title : PNAS
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 103 Sequence Number: - Start / End Page: 13789 - 13794 Identifier: -