English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  R-form LPS, the master key to the activation of TLR4/MD-2-positive cells

Huber, M., Kalis, C., Keck, S., Jiang, Z., Georgel, P., Du, X., et al. (2006). R-form LPS, the master key to the activation of TLR4/MD-2-positive cells. European Journal of Immunology, 36, 701-711.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Huber, Michael1, Author              
Kalis, Christoph2, Author              
Keck, Simone2, Author              
Jiang, Zhengfan, Author
Georgel, Philippe, Author
Du, Xin, Author
Shamel, Louis, Author
Sovath, Sosathya, Author
Mudd, Suzanne, Author
Beutler, Bruce, Author
Galanos, Chris2, Author              
Freudenberg, Marina A.3, Author              
Affiliations:
1Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645              
2Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243649              
3Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243647              

Content

show
hide
Free keywords: CD14; Lipopolysaccharide; Mast cells; TLR4
 Abstract: Lipopolysaccharide (endotoxin, LPS) is a major recognition marker for the detection of gram-negative bacteria by the host and a powerful initiator of the inflammatory response to infection. Using S- and R-form LPS from wild-type and R-mutants of Salmonella and E. coli, we show that R-form LPS readily activates mouse cells expressing the signaling receptor Toll-like receptor 4/myeloid differentiation protein 2 (TLR4/MD-2), while the S-form requires further the help of the LPS-binding proteins CD14 and LBP, which limits its activating capacity. Therefore, the R-form LPS under physiological conditions recruits a larger spectrum of cells in endotoxic reactions than S-form LPS. We also show that soluble CD14 at high concentrations enables CD14-negative cells to respond to S-form LPS. The presented in vitro data are corroborated by an in vivo study measuring TNF-α levels in response to injection of R- and S-form LPS in mice. Since the R-form LPS constitutes ubiquitously part of the total LPS present in all wild-type bacteria its contribution to the innate immune response and pathophysiology of infection is much higher than anticipated during the last half century.

Details

show
hide
Language(s): eng - English
 Dates: 2006
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 293444
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: European Journal of Immunology
  Alternative Title : Eur. J. Immunol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 36 Sequence Number: - Start / End Page: 701 - 711 Identifier: -