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  Postnatal isl1+ cardioblasts enter fully differentiated cardiomyocyte lineages

Laugwitz, K.-L., Moretti, A., Lam, J., Gruber, P., Chen, Y., Woodard, S., et al. (2005). Postnatal isl1+ cardioblasts enter fully differentiated cardiomyocyte lineages. Nature, 433, 647-653.

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Laugwitz, Karl-Ludwig, Author
Moretti, Alessandra, Author
Lam, Jason, Author
Gruber, Peter, Author
Chen, Yinhong, Author
Woodard, Sarah, Author
Lin, Li-Zhu, Author
Cai, Chen-Leng, Author
Lu, Min Min, Author
Reth, Michael1, Author              
Platoshyn, Oleksandr, Author
Yuan, Jason X. J., Author
Evans, Sylvia, Author
Chien, Kenneth R., Author
Affiliations:
1Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645              

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 Abstract: The purification, renewal and differentiation of native cardiac progenitors would form a mechanistic underpinning for unravelling steps for cardiac cell lineage formation, and their links to forms of congenital and adult cardiac diseases1-3. Until now there has been little evidence for native cardiac precursor cells in the postnatal heart4. Herein, we report the identification of isl1+ cardiac progenitors in postnatal rat, mouse and human myocardium. A cardiac mesenchymal feeder layer allows renewal of the isolated progenitor cells with maintenance of their capability to adopt a fully differentiated cardiomyocyte phenotype. Tamoxifen-inducible Cre/lox technology enables selective marking of this progenitor cell population including its progeny, at a defined time, and purification to relative homogeneity. Co-culture studies with neonatal myocytes indicate that isl1+ cells represent authentic, endogenous cardiac progenitors (cardioblasts) that display highly efficient conversion to a mature cardiac phenotype with stable expression of myocytic markers (25%) in the absence of cell fusion, intact Ca2+-cycling, and the generation of action potentials. The discovery of native cardioblasts represents a genetically based system to identify steps in cardiac cell lineage formation and maturation in development and disease.

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Language(s): eng - English
 Dates: 2005-02-10
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 263368
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 433 Sequence Number: - Start / End Page: 647 - 653 Identifier: -