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  Maternal β-catenin and E-cadherin in mouse development

de Vries, W. N., Evsikov, A. V., Haac, B. E., Fancher, K. S., Holbrook, A. E., Kemler, R., et al. (2004). Maternal β-catenin and E-cadherin in mouse development. Development, 131, 4435-4445.

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 Creators:
de Vries, Wilhelmine N., Author
Evsikov, Alexei V., Author
Haac, Bryce E., Author
Fancher, Karen S., Author
Holbrook, Andrea E., Author
Kemler, Rolf1, Author           
Solter, Davor2, Author           
Knowles, Barbara B., Author
Affiliations:
1Emeritus Group: Molecular Embryology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243656              
2Department of Developmental Biology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243650              

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Free keywords: Mouse; E-cadherin; β-catenin; Embryonic genome; Adhesion; Compaction
 Abstract: The oocyte to embryo transition in metazoans depends on maternal proteins and transcripts to ensure the successful initiation of development, and the correct and timely activation of the embryonic genome. We conditionally eliminated the maternal gene encoding the cell adhesion molecule E-cadherin and partially eliminated the β-catenin gene from the mouse oocyte. Oocytes lacking E-cadherin, or expressing a truncated allele of β-catenin without the N-terminal part of the protein, give rise to embryos whose blastomeres do not adhere. Blastomere adhesion is restored after translation of protein from the wild-type paternal alleles: at the morula stage in embryos lacking maternal E-cadherin, and at the late four-cell stage in embryos expressing truncated β-catenin. This suggests that adhesion per se is not essential in the early cleavage stage embryos, that embryos develop normally if compaction does not occur until the morula stage, and that the zona pellucida suffices to maintain blastomere proximity. Although maternal E-cadherin is not essential for the completion of the oocyte-to-embryo transition, absence of wild-type β-catenin in oocytes does statistically compromise developmental success rates. This developmental deficit is alleviated by the simultaneous absence of maternal E-cadherin, suggesting that E-cadherin regulates nuclear β-catenin availability during embryonic genome activation.

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Language(s): eng - English
 Dates: 2004
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 208483
 Degree: -

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Title: Development
Source Genre: Journal
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Pages: - Volume / Issue: 131 Sequence Number: - Start / End Page: 4435 - 4445 Identifier: -