English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Expression of housekeeping and immunoproteasome subunit genes is differentially regulated in positively and negatively selecting thymic stroma subsets

Nil, A., Firat, E., Sobek, V., Eichmann, K., & Niedermann, G. (2004). Expression of housekeeping and immunoproteasome subunit genes is differentially regulated in positively and negatively selecting thymic stroma subsets. European Journal of Immunology, 34, 2681-2689.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Nil, Ahmed1, Author              
Firat, Elke2, Author              
Sobek, Vera3, Author              
Eichmann, Klaus4, Author              
Niedermann, Gabriele3, Author              
Affiliations:
1Emeritus Group: Molecular Embryology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243656              
2Georges Köhler Laboratory, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243653              
3Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243649              
4Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              

Content

show
hide
Free keywords: Thymic selection; Antigen processing; T cell development; T cell repertoire; Proteolysis
 Abstract: The expression of housekeeping and/or immunoproteasomes in isolated thymic stroma subsets has so far not been analyzed but may have important consequences for self peptide repertoires presented by MHC class I molecules during positive and negative thymic selection. Here we determined the expression of housekeeping and immunoproteasome β subunits and of PA28 in positively and negatively selecting stroma subsets. Positively selecting cortical thymic epithelial cells (cTEC) expressed only housekeeping but no immunoproteasome β subunit mRNA and proteins. However, immunoproteasome β subunits could be induced in cTEC by infection with Listeria monocytogenes or injection of IFN-γ. In negatively selecting stroma including medullary epithelial cells and dendritic cells, incomplete and low representation of housekeeping β subunit proteins but high and complete expression of immunoproteasome β subunit proteins suggests absence of proper housekeeping proteasomes and predominance of immunoproteasomes. Expression of immunoproteasome β subunits in negatively selecting stroma was independent of IFN-γ receptor as shown in knockout (KO) mice. Absence of LMP2 altered thymic selection of the MHC class I-restricted transgenic P14 TCR in KO mice. The data suggest that negative selection may primarily involve immunoproteasome peptide repertoires and that peripheral infection may influence peptide repertoires involved in positive selection.

Details

show
hide
Language(s): eng - English
 Dates: 2004
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 214807
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: European Journal of Immunology
  Alternative Title : Eur. J. Immunol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 34 Sequence Number: - Start / End Page: 2681 - 2689 Identifier: -