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  PIASy-Deficient Mice Display Modest Deffects in IFN and Wnt Signaling

Roth, W., Sustmann, C., Kieslinger, M., Gilmozzi, A., Irmer, D., Kremmer, E., et al. (2004). PIASy-Deficient Mice Display Modest Deffects in IFN and Wnt Signaling. The Journal of Immunology, 173, 6189-6199. doi:10.4049/jimmunol.173.10.6189.

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Roth 2004_J Immunol.pdf (Publisher version), 730KB
 
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https://www.jimmunol.org/content/173/10/6189 (Publisher version)
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 Creators:
Roth, Wera1, Author
Sustmann, Claudio1, Author              
Kieslinger, Matthias1, Author              
Gilmozzi, Andrea1, Author
Irmer, Denis1, Author
Kremmer, Elisabeth1, Author
Turck, Chris1, Author
Grosschedl, Rudolf2, Author              
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1External Organizations, ou_persistent22              
2Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              

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 Abstract: Protein inhibitors of activated STATs (PIAS) represent a small family of nuclear proteins that modulate the activity of many transcription factors and act as E3 ligases for covalent modification of proteins with the small ubiquitin-like modifier (SUMO). In particular, PIASy has been shown to inhibit the activation of gene expression by the IFN-responsive transcription factor STAT1 and the Wnt-responsive transcription factor LEF1. To assess the function of PIASy in vivo, we generated and analyzed mice carrying a targeted mutation of the Piasy gene. We find that homozygous mutant mice have no obvious morphological defects and have a normal distribution of lymphocyte populations. Molecular analysis of signaling in response to IFN-γ and Wnt agonists revealed a modest reduction in the activation of endogenous and transfected target genes. Two-dimensional analysis of total proteins and SUMO-modified proteins in transformed pre-B cells showed no significant differences between wild-type mice and homozygous mutant mice. Taken together, our data indicate that PIASy has a modest effect on cytokine and Wnt signaling, suggesting a redundancy with other members of the family of PIAS proteins.

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Language(s): eng - English
 Dates: 2004-11-15
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.4049/jimmunol.173.10.6189
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Title: The Journal of Immunology
  Other : J. Immunol.
Source Genre: Journal
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Publ. Info: Baltimore, U.S.A. : Williams & Wilkins
Pages: - Volume / Issue: 173 Sequence Number: - Start / End Page: 6189 - 6199 Identifier: ISSN: 0022-1767
CoNE: https://pure.mpg.de/cone/journals/resource/954925414915_1