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  N-terminal destruction signals lead to rapid degradation of the major histocompatibility complex class II transactivator CIITA

Schnappauf, F., Hake, S. B., Camacho Carvajal, M. M., Bontron, S., Lisowska-Grospierre, B., & Steimle, V. (2003). N-terminal destruction signals lead to rapid degradation of the major histocompatibility complex class II transactivator CIITA. European Journal of Immunology, 33(8), 2337-2347.

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Schnappauf, Felix1, Author           
Hake, Sandra B.2, Author           
Camacho Carvajal, Margarita M.3, Author
Bontron, Séverine, Author
Lisowska-Grospierre, Barbara, Author
Steimle, Viktor2, Author           
Affiliations:
1Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              
2Spemann Laboratory, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243655              
3Max Planck Society, ou_persistent13              

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 Abstract: Major histocompatibility complex (MHC) class II molecules play an essential role for the cellular immune response by presenting peptide antigens to CD4+ T cells. MHC class II molecules and genes show a highly complex expression pattern, which is orchestrated through a master regulatory factor, called CIITA (class II transactivator). CIITA controls MHC class II expression not only qualitatively, but also quantitatively, and has therefore a direct influence on the CD4 T cell-dependent immune response. CIITA is itself tightly regulated not only on the transcriptional level, but as we show here also on the protein level. CIITA is subjected to a very rapid protein turnover and shows a half-life of about 30 min. Inhibition of degradation by proteasome inhibitors and the identification of ubiquitylated CIITA intermediates indicate that the degradation of CIITA is mediated by the ubiquitin-proteasome system. We identified two regions mediating degradation within the N-terminal domain of CIITA. N-terminal fusions or deletions stabilized CIITA, indicating that the N termini contribute to degradation. Several non-functional CIITA mutants are partially stabilized, but we provide evidence that transcriptional activity of CIITA is not directly linked to degradation.

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Language(s): eng - English
 Dates: 2003-08
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 126762
ISI: 000184648700031
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Title: European Journal of Immunology
  Alternative Title : Eur. J. Immunol.
Source Genre: Journal
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Pages: - Volume / Issue: 33 (8) Sequence Number: - Start / End Page: 2337 - 2347 Identifier: ISSN: 0014-2980