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  A striking correlation between lethal activity and apoptotic DNA fragmentation of liver in response of D-galactosamine- sensitized mice to a non-lethal amount of lipopolysaccharide

Zhou, B.-R., Gumenscheimer, M., Freudenberg, M., & Galanos, C. (2003). A striking correlation between lethal activity and apoptotic DNA fragmentation of liver in response of D-galactosamine- sensitized mice to a non-lethal amount of lipopolysaccharide. Acta Pharmacologica Sinica, 24(3), 193-198.

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 Creators:
Zhou, Bing-Rong, Author
Gumenscheimer, Marina1, Author              
Freudenberg, Marina2, Author              
Galanos, Chris3, Author              
Affiliations:
1Metchnikoff Laboratory, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243654              
2Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243647              
3Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243649              

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Free keywords: lipopolysaccharides; D-galactosamine; apoptosis; DNA fragmentation
 Abstract: AIM: To observe whether challenge of bacterial lipopolysaccharide (LPS) with D-galactosamine (D-GalN) in mice will result in apoptotic characteristic of vital organs. METHODS: The experimental group of mice was challenged directly with bacterial LPS (0.05 μg) in the presence of D-GalN (20 mg), and the control group of mice was challenged either with bacterial LPS or with D-GalN alone. The organs including brain, kidney, heart, spleen, lung, and liver were removed at an indicated time point under ether anethesia, and immediately homogenized, from which DNA was extracted. The DNA obtained from these organs was analyzed by agarose gel electrophoresis to determine whether the DNA laddering phenomenon existed. The amount of plasma LDH and GOT was detected in mice challenged with bacterial LPS in the presence of D-GalN, and either bacterial LPS or D-GalN alone. RESULTS: Apoptotic DNA fragmentation was initially seen at 4 h after challenge only in the livers of mice challenged with bacterial LPS and D-GalN, all mice in this group challenged with bacterial LPS and D-GalN died at 7 h after challenge; in contrast, the animals in the control group were all alive and the DNA was integral. CONCLUSION: The liver is the only specific target organ where apoptotic DNA fragmentation was present in mice treated with D- GalN and challenged with bacterial LPS and the liver impairment might be the critical cause of the lethality of mice elicited by bacterial LPS.

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Language(s): eng - English
 Dates: 2003-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 28939
ISI: 000181439200001
 Degree: -

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Title: Acta Pharmacologica Sinica
  Alternative Title : Acta Pharmacol. Sin.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 24 (3) Sequence Number: - Start / End Page: 193 - 198 Identifier: ISSN: 1671-4083