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  Toll-like receptor 4 expression levels determine the degree of LPS-susceptibility in mice

Kalis, C., Kanzler, B., Lembo, A., Poltorak, A., Galanos, C., & Freudenberg, M. A. (2003). Toll-like receptor 4 expression levels determine the degree of LPS-susceptibility in mice. European Journal of Immunology, 33(3), 798-805.

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 Creators:
Kalis, Christoph1, Author              
Kanzler, Benoit1, Author              
Lembo, Annalisa1, Author              
Poltorak, Alexander1, Author              
Galanos, Chris1, Author              
Freudenberg, Marina A.2, Author              
Affiliations:
1Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243649              
2Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243647              

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 Abstract: C57BL/10ScCr (Cr) mice carry a deletion of the Toll-like receptor 4 (tlr4) gene (i.e. they are tlr40/0 and are thus refractory to LPS effects. Insertion of wild-type tlr4 transgene into the tlr40/0 Cr germ line endowed LPS susceptibility in the two transgenic lines created, indicating that TLR4 is the only limiting factor for LPS responsiveness in Cr mice. The absolute levels of tlr4 mRNA expressed by the heterozygous transgenic (tlr4Tr/0), wild-type C57BL/10ScSn (Sn) (tlr4+/+) and heterozygous F1 (Sn x Cr) (tlr4+/0) mice varied markedly. However, the pattern of distribution of expression in the different organs was the same in all strains. In different biological assays (B cell mitogenicity, cytokine induction and lethal toxicity) the degree of LPS response obtained in the different strains of mice correlated with the levels of tlr4 mRNA expression. In macrophages, investigation of the LPS-induced cytokine (IL-6) response revealed a linear relationship between the response and the logarithm of TLR4-MD-2 levels.

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Language(s): eng - English
 Dates: 2003-03
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 126304
ISI: 000181576200023
 Degree: -

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Title: European Journal of Immunology
  Alternative Title : Eur. J. Immunol.
Source Genre: Journal
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Pages: - Volume / Issue: 33 (3) Sequence Number: - Start / End Page: 798 - 805 Identifier: ISSN: 0014-2980