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  E-cadherin is a survival factor for the lactating mouse mammary gland

Boussadia, O., Kutsch, S., Hierholzer, A., Delmas, V., & Kemler, R. (2002). E-cadherin is a survival factor for the lactating mouse mammary gland. Mechanisms of Development, 115(1-2), 53-62.

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Boussadia, Oréda, Author
Kutsch, Stefanie1, Author              
Hierholzer, Andreas1, Author              
Delmas, Véronique, Author
Kemler, Rolf1, Author              
Affiliations:
1Emeritus Group: Molecular Embryology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243656              

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Free keywords: E-cadherin cell adhesion molecule; epithelial cell differentiation; mammary gland; apoptosis; conditional gene inactivation
 Abstract: The Ca++-dependent cell adhesion molecule E-cadherin is expressed throughout mouse development and in adult tissues. Classical gene targeting has demonstrated that E-cadherin- deficient embryos die at the blastocyst stage. To study the involvement of E-cadherin in organogenesis, a conditional gene inactivation scheme was under-taken using the bacteriophage P1 recombinase Cre/loxP system. Mice with homozygous loxP sites in both alleles of the E-cadherin (Cdh1) gene were generated and these mice were crossed with transgenic mice with the Cre recombinase under the control of the hormone-inducible MMTV promoter. This resulted in deletion of the E-cadherin gene in the differentiating alveolar epithelial cells of the mammary gland. The mutant mammary gland developed normally up to 16-18 days of pregnancy but exhibited a dramatic phenotype around parturition. The production of milk proteins was so drastically reduced that adult mutant mothers could not suckle their offspring. Thus, the lack of E-cadherin affected the terminal differentiation program of the lactating mammary gland. In concordance with this finding, the prolactin-dependent activation of the transcription factor Stat5a was initiated but not maintained in the mutant gland. Instead, without E-cadherin massive cell death was observed at parturition and the mutant mammary gland at this stage resembled that of the involuted gland normally seen after weaning. These results demonstrate an essential role for E-cadherin in the function of differentiated alveolar epithelial cells. No tumors were detected in mutant glands lacking E-cadherin. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

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Language(s): eng - English
 Dates: 2002-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 20882
ISI: 000176530100006
 Degree: -

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Title: Mechanisms of Development
  Alternative Title : Mech. Dev.
Source Genre: Journal
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Pages: - Volume / Issue: 115 (1-2) Sequence Number: - Start / End Page: 53 - 62 Identifier: ISSN: 0925-4773