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  An in vitro model system for cytoskeletal confinement

Köster, S., & Pfohl, T. (2009). An in vitro model system for cytoskeletal confinement. Cell Motility and the Cytoskeleton, 66(10), 771-776. doi:10.1002/cm.20336.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-809C-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-809D-A
Genre: Journal Article

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 Creators:
Köster, Sarah1, Author              
Pfohl, Thomas1, Author              
Affiliations:
1Group Dynamics of biological matter, Department of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063313              

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 Abstract: The motility, shape, and functionality of the cell depend sensitively on cytoskeletal mechanics which in turn is governed by the properties of filamentous proteins -mainly actin, microtubules, and intermediate filaments. These biopolymers are confined in the dense cytoplasm and therefore experience strong geometric constraints on their equilibrium thermal fluctuations. To obtain a better understanding of the influence of confinement on cytoskeletal filaments we study the thermal fluctuations of individual actin filaments in a microfluidic in vitro system by fluorescence microscopy and determine the persistence length of the filaments by analyzing the radial distribution function. A unique feature of this method is that we obtain the persistence length without detailed knowledge of the complete contour of the filament which makes the technique applicable to a broad range of biological polymers, including those with a persistence length smaller than the optical resolution.

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Language(s): eng - English
 Dates: 2009-10
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: DOI: 10.1002/cm.20336
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Title: Cell Motility and the Cytoskeleton
Source Genre: Journal
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Publ. Info: New York, NY : Liss
Pages: - Volume / Issue: 66 (10) Sequence Number: - Start / End Page: 771 - 776 Identifier: ISSN: 0886-1544
CoNE: /journals/resource/954927636467