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  Modulating Vesicle Adhesion by Electric Fields

Steinkühler, J., Agudo-Canalejo, J., Lipowsky, R., & Dimova, R. (2016). Modulating Vesicle Adhesion by Electric Fields. Biophysical Journal, 111(7), 1454-1464. doi:10.1016/j.bpj.2016.08.029.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-B685-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-B686-D
Genre: Journal Article

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 Creators:
Steinkühler, Jan1, Author              
Agudo-Canalejo, Jaime2, Author              
Lipowsky, Reinhard2, Author              
Dimova, Rumiana1, Author              
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              
2Reinhard Lipowsky, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863327              

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Free keywords: Open Access
 Abstract: We introduce an experimental setup for modulating adhesion of giant unilamellar vesicles to a planar substrate. Adhesion is induced by the application of an external potential to a transparent indium tin oxide-coated electrode (the substrate), which enables single-vesicle studies. We demonstrate tunable and reversible adhesion of negatively charged vesicles. The adhesion energy at different potentials is calculated from the vesicle shape assessed with confocal microscopy. Two approaches for these estimates are employed: one based on the whole contour of the vesicle and a second based on the contact curvature of the membrane in the vicinity of the substrate. Both approaches agree well with each other and show that the adhering vesicles are in the weak adhesion regime for the range of explored external potentials. Using fluorescence quenching assays, we detect that, in the adhering membrane segment, only the outer bilayer leaflet of the vesicle is depleted of negatively charged fluorescent lipids, while the inner leaflet remains unaffected. We show that depletion of negatively charged lipids is consistent Poisson-Boltzmann theory, taking into account charge regulation from lipid mobility. Finally, we also show that lipid diffusion is not significantly affected in the adhering membrane segment. We believe that the approaches introduced here for modulating and assessing vesicle adhesion have many potential applications in the field of single-vesicle studies and research on membrane adhesion.

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 Dates: 2016-10-04
 Publication Status: Published in print
 Pages: -
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 Rev. Method: -
 Identifiers: DOI: 10.1016/j.bpj.2016.08.029
BibTex Citekey: Steinkühler20161454
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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 111 (7) Sequence Number: - Start / End Page: 1454 - 1464 Identifier: ISSN: 0006-3495