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  Nitrite is the driver, phytohormones are modulators while NO and H2O2 act as promoters of NO2-induced cell death

Kasten, D., Mithöfer, A., Georgii, E., Lang, H., Durner, J., & Gaupels, F. (2016). Nitrite is the driver, phytohormones are modulators while NO and H2O2 act as promoters of NO2-induced cell death. Journal of Experimental Botany, 67(22), 6337-6349. doi:10.1093/jxb/erw401.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-9901-A Version Permalink: https://hdl.handle.net/21.11116/0000-0009-4B5B-E
Genre: Journal Article

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BOL664s1.pdf (Supplementary material), 245KB
 
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 Creators:
Kasten, Dörte, Author
Mithöfer, Axel1, 2, Author           
Georgii, Elisabeth, Author
Lang, Hans, Author
Durner, Jörg, Author
Gaupels, Frank, Author
Affiliations:
1Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              
2Research Group Dr. A. Mithöfer, Plant Defense Physiology, Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421907              

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 Abstract: This study aimed to understand the molecular mechanisms of nitrogen dioxide (NO2)-induced toxicity and cell death in plants. Exposure of Arabidopsis to high concentrations of NO2 induced cell death in a dose-dependent manner. No leaf symptoms were visible after fumigation for 1 h with 10 parts per million (ppm) NO2. However, 20 ppm NO2 caused necrotic lesion formation and 30 ppm NO2 complete leaf collapse, which had already started during the 1 h fumigation period. NO2 fumigation resulted in a massive accumulation of nitrite and in protein modifications by S-nitrosylation and tyrosine nitration. Nitric oxide (NO) at 30 ppm did not trigger leaf damage or any of the effects observed after NO2 fumigation. The onset of NO2-induced cell death correlated with NO and hydrogen peroxide (H2O2) signaling and a decrease in antioxidants. NO- and H2O2-accumulating mutants were more sensitive to NO2 than wild-type plants. Accordingly, experiments with specific scavengers confirmed that NO and H2O2 are essential promoters of NO2-induced cell death. Leaf injection of 100 mM nitrite caused an increase in S-nitrosylation, NO, H2O2, and cell death suggesting that nitrite functioned as a mediator of NO2-induced effects. A targeted screening of phytohormone mutants revealed a protective role of salicylic acid (SA) signaling in response to NO2. It was also shown that phytohormones were modulators rather than inducers of NO2-induced cell death. The established experimental set-up is a suitable system to investigate NO2 and cell death signaling in large-scale mutant screens.

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 Dates: 2016-102016-11-02
 Publication Status: Published online
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 Identifiers: Other: BOL664
DOI: 10.1093/jxb/erw401
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Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 67 (22) Sequence Number: - Start / End Page: 6337 - 6349 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883