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  Optical imaging of individual biomolecules in densely packed clusters

Dai, M., Jungmann, R., & Yin, P. (2016). Optical imaging of individual biomolecules in densely packed clusters. Nature Nanotechnology, 11(9), 798-807. doi:10.1038/NNANO.2016.95.

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 Urheber:
Dai, Mingjie1, Autor
Jungmann, Ralf2, Autor           
Yin, Peng1, Autor
Affiliations:
1external, ou_persistent22              
2Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Schlagwörter: STRUCTURED-ILLUMINATION MICROSCOPY; FLUORESCENCE MICROSCOPY; SUPERRESOLUTION MICROSCOPY; LOCALIZATION MICROSCOPY; DNA ORIGAMI; SUBDIFFRACTION-RESOLUTION; PROTEINS; BINDING; CELLS; LIMITScience & Technology - Other Topics; Materials Science;
 Zusammenfassung: Recent advances in fluorescence super-resolution microscopy have allowed subcellular features and synthetic nanostructures down to 10-20 nm in size to be imaged. However, the direct optical observation of individual molecular targets (similar to 5 nm) in a densely packed biomolecular cluster remains a challenge. Here, we show that such discrete molecular imaging is possible using DNA-PAINT (points accumulation for imaging in nanoscale topography) a super-resolution fluorescence microscopy technique that exploits programmable transient oligonucleotide hybridization on synthetic DNA nanostructures. We examined the effects of a high photon count, high blinking statistics and an appropriate blinking duty cycle on imaging quality, and developed a software-based drift correction method that achieves <1 nm residual drift (root mean squared) over hours. This allowed us to image a densely packed triangular lattice pattern with similar to 5 nm point-to-point distance and to analyse the DNA origami structural offset with angstrom-level precision (2 A) from single-molecule studies. By combining the approach with multiplexed exchange-PAINT imaging, we further demonstrated an optical nanodisplay with 5 x 5 nm pixel size and three distinct colours with <1 nm cross-channel registration accuracy.

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Sprache(n): eng - English
 Datum: 2016-07-042016
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
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 Art der Begutachtung: -
 Identifikatoren: ISI: 000383412800015
DOI: 10.1038/NNANO.2016.95
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Titel: Nature Nanotechnology
  Andere : Nat. Nanotechnol.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: London : Nature Publishing Group
Seiten: - Band / Heft: 11 (9) Artikelnummer: - Start- / Endseite: 798 - 807 Identifikator: ISSN: 1748-3387
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000239770