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  Actin Organization in Cells Responding to a Perforated Surface, Revealed by Live Imaging and Cryo-Electron Tomography

Jasnin, M., Ecke, M., Baumeister, W., & Gerisch, G. (2016). Actin Organization in Cells Responding to a Perforated Surface, Revealed by Live Imaging and Cryo-Electron Tomography. Structure, 24(7), 1031-1043. doi:10.1016/j.str.2016.05.004.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-1145-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-1146-0
Genre: Journal Article

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 Creators:
Jasnin, Marion1, Author              
Ecke, Mary2, Author              
Baumeister, Wolfgang1, Author              
Gerisch, Günther2, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              

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Free keywords: ARP2/3 COMPLEX; LISTERIA-MONOCYTOGENES; SELF-ORGANIZATION; MOTILE CELLS; DICTYOSTELIUM; DYNAMICS; MICROSCOPY; WAVES; PROTEIN; MYOSINBiochemistry & Molecular Biology; Biophysics; Cell Biology;
 Abstract: In a 3D environment, motile cells accommodate their protruding and retracting activities to geometrical cues. Dictyostelium cells migrating on a perforated film explored its holes by forming actin rings around their border and extending protrusions through the free space. The response was initiated when an actin wave passed a hole, and the rings persisted only in the PIP3-rich territories surrounded by a wave. To reconstruct actin structures from cryo-electron tomograms, actin rings were identified by cryo-correlative light and electron microscopy, and thin wedges of relevant regions were obtained by cryo-focused ion-beam milling. Retracting stages were distinguished from protruding ones by the accumulation of myosin-II. Early actin rings consisted of filaments pointing upright from the membrane, entangled with a meshwork of filaments close to the membrane. Branches identified at later stages suggested that formin-based nucleation of filaments was followed by Arp2/3-mediated network stabilization, which prevented buckling of the force-generating filaments.

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Language(s): eng - English
 Dates: 2016-06-162016
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000383243800003
DOI: 10.1016/j.str.2016.05.004
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Title: Structure
  Other : Structure
Source Genre: Journal
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Publ. Info: London : Cell Press
Pages: - Volume / Issue: 24 (7) Sequence Number: - Start / End Page: 1031 - 1043 Identifier: ISSN: 0969-2126
CoNE: /journals/resource/954927002244_1