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  Autophagy in the test tube: In vitro reconstitution of aspects of autophagosome biogenesis

Rao, Y., Matscheko, N., & Wollert, T. (2016). Autophagy in the test tube: In vitro reconstitution of aspects of autophagosome biogenesis. FEBS Journal, 283(11), 2034-2043. doi:10.1111/febs.13661.

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 Creators:
Rao, Yijian1, Author              
Matscheko, Nena1, Author              
Wollert, Thomas1, Author              
Affiliations:
1Wollert, Thomas / Molecular Membrane and Organelle Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565175              

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Free keywords: VACUOLE TARGETING PATHWAY; PHOSPHATIDYLINOSITOL 3-KINASE; SELECTIVE AUTOPHAGY; MAMMALIAN AUTOPHAGY; SNARE PROTEINS; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; RECYCLING ENDOSOMES; CONJUGATION SYSTEM; MEMBRANE-BINDINGBiochemistry & Molecular Biology; autophagy; cellular recycling; in vitro reconstitution; membrane biology; trafficking;
 Abstract: Autophagy is a versatile recycling pathway that delivers cytoplasmic contents to lysosomal compartments for degradation. It involves the formation of a cup-shaped membrane that expands to capture cargo. After the cargo has been entirely enclosed, the membrane is sealed to generate a double-membrane-enclosed compartment, termed the autophagosome. Depending on the physiological state of the cell, the cargo is selected either specifically or non-specifically. The process involves a highly conserved set of autophagy-related proteins. Reconstitution of their action on model membranes in vitro has contributed tremendously to our understanding of autophagosome biogenesis. This review will focus on various in vitro techniques that have been employed to decipher the function of the autophagic core machinery.

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Language(s): eng - English
 Dates: 2016-02-062016
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000381316600003
DOI: 10.1111/febs.13661
 Degree: -

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Title: FEBS Journal
  Other : FEBS J.
Source Genre: Journal
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Publ. Info: Oxford, UK : Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies
Pages: - Volume / Issue: 283 (11) Sequence Number: - Start / End Page: 2034 - 2043 Identifier: ISSN: 1742-464X
CoNE: https://pure.mpg.de/cone/journals/resource/954925398485