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  Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages

Claus, V., Jahraus, A., Tjelle, T., Berg, T., Kirschke, H., Faulstich, H., et al. (1998). Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages. The Journal of Biological Chemistry, 273(16), 9842-9851. doi:10.1074/jbc.273.16.9842.

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Genre: Journal Article
Alternative Title : Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages

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JBiolChem_273_1998_9842.pdf (Any fulltext), 366KB
 
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 Creators:
Claus, Volker1, Author              
Jahraus, Andrea, Author
Tjelle, Torunn, Author
Berg, Trond, Author
Kirschke, Heidrun, Author
Faulstich, Heinz2, Author              
Griffiths, Gareth, Author
Affiliations:
1Emeritus Group Bioorganic Chemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497711              
2Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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 Abstract: In this study we take advantage of recently developed methods using J774 macrophages to prepare enriched fractions of early endosomes, late endosomes, dense lysosomes, as well as phagosomes of different ages enclosing 1-μm latex beads to investigate the steady state distribution and trafficking of lysosomal enzyme activity between these organelles. At steady state these cells appear to possess four different cellular structures, in addition to phagolysosomes, where acid hydrolases were concentrated. The first site of hydrolase concentration was the early endosomes, which contained the bulk of the cellular cathepsin H. This enzyme was acquired by phagosomes significantly faster than the other hydrolases tested. The second distinct site of lysosomal enzyme concentration was the late endosomes which contain the bulk of cathepsin S. The third and fourth large pools of hydrolases were found in two functionally distinct types of dense lysosomes, only one of which was found to be secreted in the presence of chloroquine or bafilomycin. Among this secreted pool was soluble furin, generally considered only as a membrane-bound trans-Golgi network resident protein. Thus, the organelles usually referred to as “lysosomes” in fact encompass a growing family of highly dynamic but functionally distinct endocytic organelles.

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Language(s): eng - English
 Dates: 1997-07-081998-02-021998-05-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: The Journal of Biological Chemistry
  Other : JBC
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 273 (16) Sequence Number: - Start / End Page: 9842 - 9851 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1