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  SOFI simulation tool: A software package for simulating and testing super-resolution optical fluctuation imaging.

Girsault, A., Lukes, T., Sharipov, A., Geissbuehler, S., Leutenegger, M., Vandenberg, W., et al. (2016). SOFI simulation tool: A software package for simulating and testing super-resolution optical fluctuation imaging. PLoS One, 11(9): e0161602. doi:10.1371/journal.pone.0161602.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-A2D6-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-A2DC-6
Genre: Journal Article

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 Creators:
Girsault, A., Author
Lukes, T., Author
Sharipov, A., Author
Geissbuehler, S., Author
Leutenegger, M.1, Author              
Vandenberg, W., Author
Dedecker, P., Author
Hofkens, J., Author
Lasser, T., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Super-resolution optical fluctuation imaging (SOFI) allows one to perform sub-diffraction fluorescence microscopy of living cells. By analyzing the acquired image sequence with an advanced correlation method, i.e. a high-order cross-cumulant analysis, super-resolution in all three spatial dimensions can be achieved. Here we introduce a software tool for a simple qualitative comparison of SOFI images under simulated conditions considering parameters of the microscope setup and essential properties of the biological sample. This tool incorporates SOFI and STORM algorithms, displays and describes the SOFI image processing steps in a tutorial-like fashion. Fast testing of various parameters simplifies the parameter optimization prior to experimental work. The performance of the simulation tool is demonstrated by comparing simulated results with experimentally acquired data.

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Language(s): eng - English
 Dates: 2016-09-01
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0161602
 Degree: -

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Title: PLoS One
Source Genre: Journal
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Pages: 13 Volume / Issue: 11 (9) Sequence Number: e0161602 Start / End Page: - Identifier: -