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  The extracellular human melanoma inhibitory activity (MIA) protein adopts an SH3 domain-like fold.

Stoll, R., Renner, C., Zweckstetter, M., Brüggert, M., Ambrosius, D., Palme, S., et al. (2001). The extracellular human melanoma inhibitory activity (MIA) protein adopts an SH3 domain-like fold. EMBO Journal, 20(3), 340-349. doi:10.1093%2Femboj%2F20.3.340.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-A5A8-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-A5AB-B
Genre: Journal Article

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2353735.pdf (Publisher version), 417KB
 
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 Creators:
Stoll, R., Author
Renner, C., Author
Zweckstetter, M.1, Author              
Brüggert, M., Author
Ambrosius, D., Author
Palme, S., Author
Engh, R. A., Author
Golob, M., Author
Breibach, I., Author
Buettner, R., Author
Voelter, W., Author
Holak, T. A., Author
Bosserhoff, A. K., Author
Affiliations:
1Research Group of Protein Structure Determination using NMR, MPI for biophysical chemistry, Max Planck Society, ou_578571              

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 Abstract: Melanoma inhibitory activity (MIA) protein is a clinically valuable marker in patients with malignant melanoma, as enhanced values diagnose metastatic melanoma stages III and IV. Here we show that the recombinant human MIA adopts an SH3 domain-like fold in solution, with two perpendicular, antiparallel, three- and five-stranded beta-sheets. In contrast to known structures with the SH3 domain fold, MIA is a single-domain protein, and contains an additional antiparallel beta-sheet and two disulfide bonds. MIA is also the first extracellular protein found to have the SH3 domain-like fold. Furthermore, we show that MIA interacts with fibronectin and that the peptide ligands identified for MIA exhibit a matching sequence to type III human fibronectin repeats, especially to FN14, which is close to an integrin alpha4beta1 binding site. The present study, therefore, may explain the role of MIA in metastasis in vivo, and supports a model in which the binding of human MIA to type III repeats of fibronectin competes with integrin binding, thus detaching cells from the extracellular matrix.

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Language(s): eng - English
 Dates: 2001-02-01
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1093%2Femboj%2F20.3.340
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Title: EMBO Journal
Source Genre: Journal
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Pages: - Volume / Issue: 20 (3) Sequence Number: - Start / End Page: 340 - 349 Identifier: -