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  The phosphatase Dusp7 drives meiotic resumption and chromosome alignment in mouse oocytes.

Tischer, T., & Schuh, M. (2016). The phosphatase Dusp7 drives meiotic resumption and chromosome alignment in mouse oocytes. Cell Reports, 17(5), 1426-1437. doi:10.1016/j.celrep.2016.10.007.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-AAAC-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-273A-F
Genre: Journal Article

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 Creators:
Tischer, T., Author
Schuh, M.1, Author              
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

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Free keywords: DUSP7; PKC; chromosome segregation; meiosis; mouse oocytes; phosphatase; prophase arrest
 Abstract: Mammalian oocytes are stored in the ovary, where they are arrested in prophase for prolonged periods. The mechanisms that abrogate the prophase arrest in mammalian oocytes and reinitiate meiosis are not well understood. Here, we identify and characterize an essential pathway for the resumption of meiosis that relies on the protein phosphatase DUSP7. DUSP7-depleted oocytes either fail to resume meiosis or resume meiosis with a significant delay. In the absence of DUSP7, Cdk1/CycB activity drops below the critical level required to reinitiate meiosis, precluding or delaying nuclear envelope breakdown. Our data suggest that DUSP7 drives meiotic resumption by dephosphorylating and thereby inactivating cPKC isoforms. In addition to controlling meiotic resumption, DUSP7 has a second function in chromosome segregation: DUSP7-depleted oocytes that enter meiosis show severe chromosome alignment defects and progress into anaphase prematurely. Altogether, these findings establish the phosphatase DUSP7 as an essential regulator of multiple steps in oocyte meiosis.

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Language(s): eng - English
 Dates: 2016-10-252016-10-25
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.celrep.2016.10.007
 Degree: -

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Title: Cell Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 17 (5) Sequence Number: - Start / End Page: 1426 - 1437 Identifier: -