English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Carboxylated photoswitchable diarylethenes for biolabeling and super-resolution RESOLFT microscopy.

Roubinet, B., Bossi, M. L., Alt, P., Leutenegger, M., Shojaei, H., Schnorrenberg, S., et al. (2016). Carboxylated photoswitchable diarylethenes for biolabeling and super-resolution RESOLFT microscopy. Angewandte Chemie International Edition, 55(49), 15429-15433. doi:10.1002/anie.201607940.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-AC7B-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-1849-6
Genre: Journal Article

Files

show Files
hide Files
:
2354743.pdf (Publisher version), 3MB
Name:
2354743.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2354743_Suppl.pdf (Supplementary material), 5MB
Name:
2354743_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Roubinet, B.1, Author              
Bossi, M. L.1, Author              
Alt, P.1, Author              
Leutenegger, M.1, Author              
Shojaei, H.1, Author              
Schnorrenberg, S.1, Author              
Nizamov, S.1, Author              
Irie, M., Author
Belov, V. N.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

Content

show
hide
Free keywords: Diarylethenes; Fluorescence; Optical microscopy; Photochromic dyes; Water solubility
 Abstract: Reversibly photoswitchable 1,2-bis(2-ethyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes (EBT) having fluorescent "closed" forms were decorated with four or eight carboxylic groups and attached to antibodies. Low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and good photophysical properties were demonstrated. Alternating light pulses of UV and blue light induce numerous reversible photochemical transformations between two stables states with distinct structures. Using relatively low light intensities, EBTs were applied in biology-related super-resolution microscopy based on the reversible saturable (switchable) optical linear fluorescence transitions (RESOLFT) and demonstrated optical resolution of 75 nm.

Details

show
hide
Language(s): eng - English
 Dates: 2016-10-212016-12-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/anie.201607940
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Angewandte Chemie International Edition
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 55 (49) Sequence Number: - Start / End Page: 15429 - 15433 Identifier: -