English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles.

Fricke, P., Mance, D., Chevelkov, V., Giller, K., Becker, S., Baldus, M., et al. (2016). High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles. Journal of Biomolecular NMR, 65(3-4), 121-126. doi:10.1007/s10858-016-0044-y.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-B05B-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-ABEE-4
Genre: Journal Article

Files

show Files
hide Files
:
2355167.pdf (Publisher version), 4MB
 
File Permalink:
-
Name:
2355167.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2355167_Suppl.pdf (Supplementary material), 557KB
Name:
2355167_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Fricke, P., Author
Mance, D. , Author
Chevelkov, V., Author
Giller, K.1, Author              
Becker, S.1, Author              
Baldus, M., Author
Lange, A., Author
Affiliations:
1Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

Content

show
hide
Free keywords: Proteins; Type III Secretion System; Solid-State NMR; DNP; Dynamics; PRE
 Abstract: The cryogenic temperatures at which dynamic nuclear polarization (DNP) solid-state NMR experiments need to be carried out cause line-broadening, an effect that is especially detrimental for crowded protein spectra. By increasing the magnetic field strength from 600 to 800 MHz, the resolution of DNP spectra of type III secretion needles (T3SS) could be improved by 22 %, indicating that inhomogeneous broadening is not the dominant effect that limits the resolution of T3SS needles under DNP conditions. The outstanding spectral resolution of this system under DNP conditions can be attributed to its low overall flexibility.

Details

show
hide
Language(s): eng - English
 Dates: 2016-06-282016-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s10858-016-0044-y
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Biomolecular NMR
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 65 (3-4) Sequence Number: - Start / End Page: 121 - 126 Identifier: -