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  Translocation as continuous movement through the ribosome.

Belardinelli, R., Sharma, H., Peske, F., Wintermeyer, W., & Rodnina, M. V. (2016). Translocation as continuous movement through the ribosome. RNA Biology, 13(12), 1197-1203. doi:10.1080/15476286.2016.1240140.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-B680-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-6569-0
Genre: Journal Article

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2357891.pdf (Publisher version), 2MB
 
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 Creators:
Belardinelli, R.1, Author              
Sharma, H.1, Author              
Peske, F.1, Author              
Wintermeyer, W.2, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              
2Research Group of Ribosome Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578599              

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Free keywords: EF-G; mRNA; molecular machines; protein synthesis; ribosome; tRNA; translation; translation elongation; translocation
 Abstract: In each round of translation elongation, tRNAs and mRNA move within the ribosome by one codon at a time. tRNA-mRNA translocation is promoted by elongation factor G (EF-G) at the cost of GTP hydrolysis. The key questions for understanding translocation are how and when the tRNAs move and how EF-G coordinates motions of the ribosomal subunits with tRNA movement. Here we present 2 recent papers which describe the choreography of movements over the whole trajectory of translocation. We present the view that EF-G accelerates translocation by promoting the steps that lead to GTPase-dependent ribosome unlocking. EF-G facilitates the formation of the rotated state of the ribosome and uncouples the backward motions of the ribosomal subunits, forming an open conformation in which the tRNAs can rapidly move. Ribosome dynamics are important not only in translocation, but also in recoding events, such as frameshifting and bypassing, and mediate sensitivity to antibiotics.

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Language(s): eng - English
 Dates: 2016-11-012016
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1080/15476286.2016.1240140
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Title: RNA Biology
Source Genre: Journal
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Pages: - Volume / Issue: 13 (12) Sequence Number: - Start / End Page: 1197 - 1203 Identifier: -