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  The pathway to GTPase activation of elongation factor SelB on the ribosome.

Fischer, N., Neumann, P., Bock, l. V., Maracci, C., Wang, Z., Paleskava, A., et al. (2016). The pathway to GTPase activation of elongation factor SelB on the ribosome. Nature, 540(7631), 80-85. doi:10.1038/nature20560.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-B6A1-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-1CD2-D
Genre: Journal Article

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Fischer, N., Author
Neumann, P., Author
Bock, l. V., Author
Maracci, C.1, Author              
Wang, Z., Author
Paleskava, A., Author
Konevega, A. L.1, Author              
Schröder, G. F., Author
Grubmüller, H.2, Author              
Ficner, R., Author
Rodnina, M. V.1, Author              
Stark, H.3, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              
2Department of Theoretical and Computational Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578631              
3Department of Structural Dynamics, MPI for Biophysical Chemistry, Max Planck Society, ou_2205645              

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 Abstract: In all domains of life, selenocysteine (Sec) is delivered to the ribosome by selenocysteine-specific tRNA (tRNASec) with the help of a specialized translation factor, SelB in bacteria. Sec-tRNASec recodes a UGA stop codon next to a downstream mRNA stem-loop. Here we present the structures of six intermediates on the pathway of UGA recoding in Escherichia coli by single-particle cryo-electron microscopy. The structures explain the specificity of Sec-tRNASec binding by SelB and show large-scale rearrangements of Sec-tRNASec. Upon initial binding of SelB-Sec-tRNASec to the ribosome and codon reading, the 30S subunit adopts an open conformation with Sec-tRNASec covering the sarcin-ricin loop (SRL) on the 50S subunit. Subsequent codon recognition results in a local closure of the decoding site, which moves Sec-tRNASec away from the SRL and triggers a global closure of the 30S subunit shoulder domain. As a consequence, SelB docks on the SRL, activating the GTPase of SelB. These results reveal how codon recognition triggers GTPase activation in translational GTPases.

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Language(s): eng - English
 Dates: 2016-11-142016-12-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nature20560
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 540 (7631) Sequence Number: - Start / End Page: 80 - 85 Identifier: -