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  Functional expression of the calcium release channel from skeletal muscle ryanodine receptor cDNA.

Penner, R., Neher, E., Takeshima, H., Nishimura, S., & Numa, S. (1989). Functional expression of the calcium release channel from skeletal muscle ryanodine receptor cDNA. FEBS Letters, 259(1), 217-221. doi:10.1016/0014-5793(89)81532-7.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-B79B-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-B79C-8
Genre: Journal Article

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2358746.pdf (Publisher version), 539KB
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 Creators:
Penner, R.1, Author              
Neher, E.1, Author              
Takeshima, H., Author
Nishimura, S., Author
Numa, S., Author
Affiliations:
1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: Combined patch-clamp and fura-2 measurements were performed to study the calcium release properties of Chinese hamster ovary (CHO) cells transfected with the rabbit skeletal muscle ryanodine receptor cDNA carried by an expression vector. Both caffeine (1–50 mM) and ryanodine (100 μM) induced release of calcium from intracellular stores of transformed CHO cells but not from control (non-transfected) CHO cells. The calcium responses to caffeine and ryanodine closely resembled those commonly observed in skeletal muscle. Repetitive applications of caffeine produced characteristic all-or-none rises in intracellular calcium. Inositol 1,4,5-trisphosphate (IP3) neither activated the ryanodine receptor channel nor interfered with the caffeine-elicited calcium release. These results indicate that functional calcium release channels are formed by expression of the ryanodine receptor cDNA.

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Language(s): eng - English
 Dates: 1989-12
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/0014-5793(89)81532-7
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Title: FEBS Letters
Source Genre: Journal
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Pages: - Volume / Issue: 259 (1) Sequence Number: - Start / End Page: 217 - 221 Identifier: -