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  Adaptive wavefront correction in two-photon microscopy using coherence gated wavefront sensing

Rückel, M., Mack-Bucher, J. A., & Denk, W. (2006). Adaptive wavefront correction in two-photon microscopy using coherence gated wavefront sensing. Proceedings of the National Academy of Sciences of the United States of America (ASTOR), 103(46), 17137-17142. doi:10.1073/pnas.0604791103.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-BC90-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-BC91-7
Genre: Journal Article
Alternative Title : Adaptive wavefront correction in two-photon microscopy using coherence gated wavefront sensing

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PNAS_103_2006_17137.pdf (Any fulltext), 2MB
 
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 Creators:
Rückel, Markus1, Author              
Mack-Bucher, Julia Angela1, Author              
Denk, Winfried1, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: aberration; adaptive optics; biological tissue; deformable mirror; scattering
 Abstract: The image quality of a two-photon microscope is often degraded by wavefront aberrations induced by the specimen. We demonstrate here that resolution and signal size in two-photon microcopy can be substantially improved, even in living biological specimens, by adaptive wavefront correction based on sensing the wavefront of coherence-gated backscattered light (coherence-gated wavefront sensing, CGWS) and wavefront control by a deformable mirror. A nearly diffraction-limited focus can be restored even for strong aberrations. CGWS-based wavefront correction should be applicable to samples with a wide range of scattering properties and it should be possible to perform real-time pixel-by-pixel correction even at fast scan speeds.

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Language(s): eng - English
 Dates: 2006-06-082006-09-282006-11-062006-11-14
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 665343
DOI: 10.1073/pnas.0604791103
URI: http://www.ncbi.nlm.nih.gov/pubmed/17088565
Other: 6669
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America (ASTOR)
  Other : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Publ. Info: National Academy of Sciences
Pages: - Volume / Issue: 103 (46) Sequence Number: - Start / End Page: 17137 - 17142 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230