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  Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism

Saha, S., Weber, C. A., Nousch, M., Adame-Arana, O., Hoege, C., Hein, M. Y., et al. (2016). Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism. Cell, 166(6), 1572-1584.e16. doi:10.1016/j.cell.2016.08.006.

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Saha, Shambaditya1, Author
Weber, Christoph A.2, Author           
Nousch, Marco1, Author
Adame-Arana, Omar2, Author           
Hoege, Carsten1, Author
Hein, Marco Y.1, Author
Osborne-Nishimura, Erin1, Author
Mahamid, Julia1, Author
Jahnel, Marcus1, Author
Jawerth, Louise2, Author           
Pozniakovski, Andrej1, Author
Eckmann, Christian R.1, Author
Jülicher, Frank2, Author           
Hyman, Anthony A.1, Author
Affiliations:
1external, ou_persistent22              
2Max Planck Institute for the Physics of Complex Systems, Max Planck Society, ou_2117288              

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 MPIPKS: Living matter
 Abstract: P granules are non-membrane-bound RNA-protein compartments that are involved in germline development in C. elegans. They are liquids that condense at one end of the embryo by localized phase separation, driven by gradients of polarity proteins such as the mRNA-binding protein MEX-5. To probe how polarity proteins regulate phase separation, we combined biochemistry and theoretical modeling. We reconstitute P granule-like droplets in vitro using a single protein PGL-3. By combining in vitro reconstitution with measurements of intracellular concentrations, we show that competition between PGL-3 and MEX-5 for mRNA can regulate the formation of PGL-3 droplets. Using theory, we show that, in a MEX-5 gradient, this mRNA competition mechanism can drive a gradient of P granule assembly with similar spatial and temporal characteristics to P granule assembly in vivo. We conclude that gradients of polarity proteins can position RNP granules during development by using RNA competition to regulate local phase separation.

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 Dates: 2016-09-012016-09-08
 Publication Status: Issued
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 Identifiers: DOI: 10.1016/j.cell.2016.08.006
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Title: Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 166 (6) Sequence Number: - Start / End Page: 1572 - 1584.e16 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183