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  NMR investigations of interactions between anesthetics and lipid bilayers

Castro, V., Stevensson, B., Dvinskikh, S. V., Högbert, C.-J., Lyubartsev, A. P., Zimmermann, H., et al. (2008). NMR investigations of interactions between anesthetics and lipid bilayers. Biochimica et Biophysica Acta, 1778(11), 2604-2611. doi:10.1016/j.bbamem.2008.07.023.

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BBA_1778_2008_2604.pdf (Any fulltext), 372KB
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 Creators:
Castro, Vasco, Author
Stevensson, Baltzar, Author
Dvinskikh, Sergey V., Author
Högbert, Carl-Johan, Author
Lyubartsev, Alexander P., Author
Zimmermann, Herbert1, Author           
Sandström, Dick, Author
Maliniak, Arnold, Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: Solid-state NMR; Carbon-13; Phosphorus-31; Deuterium; Dipolar couplings; Separated local field; Quadrupolar coupling; Order parameter; Lyotropic liquid crystal; Biomembrane; Anesthetic
 Abstract: Interactions between anesthetics (lidocaine and short chain alcohols) and lipid membranes formed by dimyristoylphosphatidylcholine (DMPC) were studied using NMR spectroscopy. The orientational order of lidocaine was investigated using deuterium NMR on a selectively labelled compound whereas segmental ordering in the lipids was probed by two-dimensional 1H-13C separated local field experiments under magic-angle spinning conditions. In addition, trajectories generated in molecular dynamics (MD) computer simulations were used for interpretation of the experimental results. Separate simulations were carried out with charged and uncharged lidocaine molecules. Reasonable agreement between experimental dipolar interactions and the calculated counterparts was observed. Our results clearly show that charged lidocaine affects significantly the lipid headgroup. In particular the ordering of the lipids is increased accompanied by drastic changes in the orientation of the P-N vector in the choline group.

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Language(s): eng - English
 Dates: 2008-07-112008-04-082008-07-142008-11
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Biochimica et Biophysica Acta
Source Genre: Journal
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Publ. Info: Amsterdam [etc.] : Elsevier
Pages: - Volume / Issue: 1778 (11) Sequence Number: - Start / End Page: 2604 - 2611 Identifier: ISSN: 0006-3002
CoNE: https://pure.mpg.de/cone/journals/resource/954925384085