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  Changes in acetylcholine receptor function induce shifts in muscle fiber type composition

Jin, T.-E., Wernig, A., & Witzemann, V. (2008). Changes in acetylcholine receptor function induce shifts in muscle fiber type composition. FEBS Journal, 275(9), 2042-2054. doi:10.1111/j.1742-4658.2008.06359.x.

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FEBSJ_275_2008_2042.pdf (Any fulltext), 767KB
 
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Jin, Tae-Eun, Author
Wernig, Anton, Author
Witzemann, Veit1, Author           
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1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: acetylcholine receptor; acetylcholine receptor e−subunit knockout mice; fast and slow muscle; fiber type; real−time PCR
 Abstract: AChRepsilon(-/-) mice lack epsilon-subunits of the acetylcholine receptor and thus fail to express adult-type receptors. The expression of fetal-type receptors throughout postnatal life alters postsynaptic signal transduction and causes a fast-to-slow fiber type transition, both in slow-twitch soleus muscle and in fast-twitch extensor digitorum longus muscle. In comparison to wild-type muscle, the proportion of type 1 slow fibers is significantly increased (6%), whereas the proportion of fast fibers is reduced (in soleus, type 2A by 12%, and in extensor digitorum longus, type 2B/2D by 10%). The increased levels of troponin I(slow) transcripts clearly support a fast-to-slow fiber type transition. Shifts of protein and transcript levels are not restricted to 'myogenic' genes but also affect 'synaptogenic' genes. Clear increases are observed for acetylcholine receptor alpha-subunits and the postsynaptically located utrophin. Although the fast-to-slow fiber type transition appears to occur in a coordinated manner in both muscle types, muscle-specific differences are retained. Most prominently, the differential expression level of the synaptic regulator MuSK is significantly lower in extensor digitorum muscle than in soleus muscle. The results show a new quality in muscle plasticity, in that changes in the functional properties of endplate receptors modulate the contractile properties of skeletal muscles. Muscle thus represents a self-matching system that adjusts contractile properties and synaptic function to variable functional demands.

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Language(s): eng - English
 Dates: 2008-02-122008-01-092008-02-252008-04-012008-05
 Publication Status: Issued
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: FEBS Journal
  Other : FEBS J.
Source Genre: Journal
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Publ. Info: Oxford, UK : Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies
Pages: - Volume / Issue: 275 (9) Sequence Number: - Start / End Page: 2042 - 2054 Identifier: ISSN: 1742-464X
CoNE: https://pure.mpg.de/cone/journals/resource/954925398485