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  Photophysical properties of popular fluorescent adenosine nucleotide analogs used in enzyme mechanism probing

Leskovar, A., & Reinstein, J. (2008). Photophysical properties of popular fluorescent adenosine nucleotide analogs used in enzyme mechanism probing. Archives of Biochemistry and Biophysics, 473(1), 16-24. doi:10.1016/j.abb.2008.02.035.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-067A-A Version Permalink: http://hdl.handle.net/21.11116/0000-0000-DBF8-2
Genre: Journal Article

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ArchBiochemBiophys_473_2008_16.pdf (Any fulltext), 761KB
 
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 Creators:
Leskovar, Adriane1, Author              
Reinstein, Jochen1, Author              
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: Fluorescence, Nucleotide, Mechanism, Probe, Kinetics, Chaperone
 Abstract: Fluorescent nucleotide analogs are widely used in mechanistic studies of nucleotide binding and utilizing proteins. We describe here an overview of the photophysical parameters of the most popular nucleotide analogs that have a fluorescent N-methylanthraniloyl-group attached at various positions of the nucleotide. Steady state absorption and fluorescence spectra of free chromophores depend on the type of modification (ribose, base or phosphate moiety) and the addition of proteins suggests that the labeled nucleotides also vary in sensitivity depending upon their local protein environment. Fluorescence lifetime measurements imply two to three lifetimes for each nucleotide with complex changes in dependence on solvent but more importantly also on the protein. The measured quantum yields quantify the increase in fluorescence for (C8)-MABA-ADP, MANT-ATP and (Pgamma)-MABA-ATP as 153%, 93% and 14% when bound to DnaK, ClpB and Trap1, respectively, compared to free in buffer solution.

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Language(s): eng - English
 Dates: 2007-11-292008-02-292008-02-292008-05-01
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Archives of Biochemistry and Biophysics
Source Genre: Journal
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Publ. Info: New York : Academic Press
Pages: - Volume / Issue: 473 (1) Sequence Number: - Start / End Page: 16 - 24 Identifier: ISSN: 0003-9861
CoNE: https://pure.mpg.de/cone/journals/resource/991042745826956