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  Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser.

Ekeberg, T., Svenda, M., Abergel, C., Maia, F. R. N. C., Seltzer, V., Claverie, J.-M., et al. (2015). Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser. Physical Review Letters, 114(9): 098102. doi:10.1103/PhysRevLett.114.098102.

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 Creators:
Ekeberg, T., Author
Svenda, M., Author
Abergel, C., Author
Maia, F. R. N. C., Author
Seltzer, V., Author
Claverie, J.-M., Author
Hantke, M., Author
Jönsson, O., Author
Nettelblad, C., Author
van der Schot, G., Author
Liang, M., Author
DePonte, D. P., Author
Barty, A., Author
Seibert, M. M., Author
Iwan, B., Author
Andersson, I., Author
Loh, N. D., Author
Martin, A. V., Author
Chapman, H., Author
Bostedt, C., Author
Bozek, J. D., AuthorFerguson, K. R., AuthorKrzywinski, J., AuthorEpp, S. W., AuthorRolles, D.1, Author              Rudenko, A., AuthorHartmann, R., AuthorKimmel, N., AuthorHajdu, J., Author more..
Affiliations:
1Research Group of Structural Dynamics of (Bio)Chemical Systems, MPI for Biophysical Chemistry, Max Planck Society, ou_578564              

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 Abstract: We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.

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Language(s): eng - English
 Dates: 2015-03-022015-03-06
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1103/PhysRevLett.114.098102
 Degree: -

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Title: Physical Review Letters
Source Genre: Journal
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Pages: 6 Volume / Issue: 114 (9) Sequence Number: 098102 Start / End Page: - Identifier: -