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  High-throughput imaging of heterogeneous cell organelles with an X-ray laser.

Hantke, M. F., Hasse, D., Maia, F. R. N. C., Ekeberg, T., John, K., Svenda, M., et al. (2014). High-throughput imaging of heterogeneous cell organelles with an X-ray laser. Nature Photonics, 8, 943-949. doi:10.1038/nphoton.2014.270.

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 Creators:
Hantke, M. F., Author
Hasse, D., Author
Maia, F. R. N. C., Author
Ekeberg, T., Author
John, K., Author
Svenda, M., Author
Loh, N. D., Author
Vartin, A., Author
Timneanu, N., Author
Larsson, D. S. D., Author
van der Schot, G., Author
Carlsson, G. H., Author
Ingelman, M., Author
Andreasson, J., Author
Westphal, D., Author
Liang, M., Author
Stellato, F., Author
DePonte, D. P., Author
Hartmann, R., Author
Kimmel, N., Author
Kirian, R. A., AuthorSeibert, M. M., AuthorMühlig, K., AuthorSchorb, S., AuthorFerguson, K., AuthorBostedt, C., AuthorCarron, S., AuthorBozek, J. D., AuthorRolles, D.1, Author              Rudenko, A., AuthorEpp, S., AuthorChapman, H. N., AuthorBarty, A., AuthorHajdu, J., AuthorAndersson, I., Author more..
Affiliations:
1Research Group of Structural Dynamics of (Bio)Chemical Systems, MPI for Biophysical Chemistry, Max Planck Society, ou_578564              

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 Abstract: We overcome two of the most daunting challenges in single-particle diffractive imaging: collecting many high-quality diffraction patterns on a small amount of sample and separating components from mixed samples. We demonstrate this on carboxysomes, which are polyhedral cell organelles that vary in size and facilitate up to 40% of Earth's carbon fixation. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min with the Linac Coherent Light Source running at 120 Hz. We separate different structures directly from the diffraction data and show that the size distribution is preserved during sample delivery. We automate phase retrieval and avoid reconstruction artefacts caused by missing modes. We attain the highest-resolution reconstructions on the smallest single biological objects imaged with an X-ray laser to date. These advances lay the foundations for accurate, high-throughput structure determination by flash-diffractive imaging and offer a means to study structure and structural heterogeneity in biology and elsewhere.

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Language(s): eng - English
 Dates: 2014-11-172014
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nphoton.2014.270
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Title: Nature Photonics
Source Genre: Journal
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Pages: - Volume / Issue: 8 Sequence Number: - Start / End Page: 943 - 949 Identifier: -