English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Remodelling of the respiratory network in a mouse model of Rett syndrome depends on brain-derived neurotrophic factor regulated slow calcium buffering

Mironov, S. L., Skorova, E., Hartelt, N., Mironova, L. A., Hasan, M. T., & Kügler, S. (2009). Remodelling of the respiratory network in a mouse model of Rett syndrome depends on brain-derived neurotrophic factor regulated slow calcium buffering. The Journal of Physiology - London, 587(11), 2473-2485. doi:10.1113/jphysiol.2009.169805.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-11B9-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-11BA-A
Genre: Journal Article
Alternative Title : Remodelling of the respiratory network in a mouse model of Rett syndrome depends on brain-derived neurotrophic factor regulated slow calcium buffering

Files

show Files
hide Files
:
JPhysiol_587_2009_2473.pdf (Any fulltext), 725KB
 
File Permalink:
-
Name:
JPhysiol_587_2009_2473.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Description:
-

Creators

show
hide
 Creators:
Mironov, S. L., Author
Skorova, E., Author
Hartelt, N., Author
Mironova, L. A., Author
Hasan, Mazahir T.1, 2, Author              
Kügler, Sebastian, Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

Content

show
hide
Free keywords: -
 Abstract: Rett syndrome caused by MeCP2 mutations is a devastating neurodevelopmental disorder accompanied by severe breathing irregularities. Using transduction of organotypic slices from model MeCP2-/y mice with neuron-specific calcium sensor protein D3cpv, we examined the slow calcium buffering in neurons in pre-Bötzinger complex (preBötC), a component of the complex respiratory network. Examination of wild-type (WT) and MeCP2 null mice showed clear differences in the spatial organisations of neurons in preBötC and also in the disturbances in calcium homeostasis in mutant mice during early postnatal development. Deregulated calcium buffering in MeCP2-/y neurons was indicated by increased amplitude and kinetics of depolarisation-induced calcium transients. Both effects were related to an insufficient calcium uptake into the endoplasmic reticulum that was restored after pretreatment with brain-derived neurotrophic factor (BNDF). Conversely, the inhibition of BDNF signalling in WT neurons produced disturbances similar to those observed in MeCP2-/y mice. Brief hypoxia and calcium release from internal stores induced global calcium increases, after which the processes of many MeCP2-/y neurons were retracted, an effect that was also corrected by pretreatment with BDNF. The data obtained point to a tight connection between calcium homeostasis and long-term changes in neuronal connectivity. We therefore propose that calcium-dependent retraction of neurites in preBötC neurons can cause remodelling of the neuronal network during development and set up the conditions for appearance of breathing irregularities in Rett model mice.

Details

show
hide
Language(s): eng - English
 Dates: 2009-01-272009-03-302009-04-092009-06-01
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Journal of Physiology - London
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Cambridge University Press
Pages: - Volume / Issue: 587 (11) Sequence Number: - Start / End Page: 2473 - 2485 Identifier: ISSN: 0022-3751
CoNE: https://pure.mpg.de/cone/journals/resource/954925334693_2