English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion

Sacco, F., Humphrey, S. J., Cox, J., Mischnik, M., Schulte, A., Klabunde, T., et al. (2016). Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nature Communications, 7: 13250. doi:10.1038/ncomms13250.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-11FF-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-2F3B-9
Genre: Journal Article

Files

show Files
hide Files
:
ncomms13250.pdf (Publisher version), 2MB
Name:
ncomms13250.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
The Author(s) 2016 This work is licensed under a Creative Commons Attribution 4.0 International License.
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Sacco, Francesca1, Author              
Humphrey, Sean J.1, Author              
Cox, Jürgen2, Author              
Mischnik, Marcel3, Author
Schulte, Anke3, Author
Klabunde, Thomas3, Author
Schaefer, Matthias3, Author
Mann, Matthias1, Author              
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_2063284              
3external, ou_persistent22              

Content

show
hide
Free keywords: PANCREATIC BETA-CELLS; IN-VIVO; SIGNALING NETWORKS; MASS-SPECTROMETRY; PROTEIN-KINASES; COPY-NUMBER; PHOSPHORYLATION; DYNAMICS; COMPLEX; MOUSEScience & Technology - Other Topics;
 Abstract: Insulin-secreting beta cells play an essential role in maintaining physiological blood glucose levels, and their dysfunction leads to the development of diabetes. To elucidate the signalling events regulating insulin secretion, we applied a recently developed phosphoproteomics workflow. We quantified the time-resolved phosphoproteome of murine pancreatic cells following their exposure to glucose and in combination with small molecule compounds that promote insulin secretion. The quantitative phosphoproteome of 30,000 sites clustered into three main groups in concordance with the modulation of the three key kinases: PKA, PKC and CK2A. A high-resolution time course revealed key novel regulatory sites, revealing the importance of methyltransferase DNMT3A phosphorylation in the glucose response. Remarkably a significant proportion of these novel regulatory sites is significantly down-regulated in diabetic islets. Control of insulin secretion is embedded in an unexpectedly broad and complex range of cellular functions, which are perturbed by drugs in multiple ways.

Details

show
hide
Language(s): eng - English
 Dates: 2016-11-14
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000387558800001
DOI: 10.1038/ncomms13250
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 13250 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: /journals/resource/2041-1723