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  Scaffold-free Tissue Formation Under Real and Simulated Microgravity Conditions

Aleshcheva, G., Bauer, J., Hemmersbach, R., Slumstrup, L., Wehland, M., Infanger, M., et al. (2016). Scaffold-free Tissue Formation Under Real and Simulated Microgravity Conditions. Basic & Clinical Pharmacology & Toxicology, 119, 26-33. doi:10.1111/bcpt.12561.

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Genre: Conference Paper
Other : MiniReview

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 Creators:
Aleshcheva, Ganna1, Author
Bauer, Johann2, Author              
Hemmersbach, Ruth1, Author
Slumstrup, Lasse1, Author
Wehland, Markus1, Author
Infanger, Manfred1, Author
Grimm, Daniela1, Author
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: PARABOLIC FLIGHT MANEUVERS; HUMAN ENDOTHELIAL-CELLS; THYROID-CANCER CELLS; DIFFERENTIAL GENE-EXPRESSION; HUMAN CHONDROCYTES; SPHEROID FORMATION; GROWTH-FACTOR; 3-DIMENSIONAL GROWTH; INCREASES APOPTOSIS; GROWING TISSUESPharmacology & Pharmacy; Toxicology;
 Abstract: Scaffold-free tissue formation in microgravity is a new method in regenerative medicine and an important topic in Space Medicine. In this MiniReview, we focus on recent findings in the field of tissue engineering that were observed by exposing cells to real microgravity in space or to devices simulating to at least some extent microgravity conditions on Earth (ground-based facilities). Under both conditions - real and simulated microgravity - a part of the cultured cells of various populations detaches from the bottom of a culture flask. The cells form three-dimensional (3D) aggregates resembling the organs from which the cells have been derived. As spaceflights are rare and extremely expensive, cell culture under simulated microgravity allows more comprehensive and frequent studies on the scaffold-free 3D tissue formation in some aspects, as a number of publications have proven during the last two decades. In this MiniReview, we summarize data from our own studies and work from various researchers about tissue engineering of multi-cellular spheroids formed by cancer cells, tube formation by endothelial cells and cartilage formation by exposing the cells to ground-based facilities such as the 3D Random Positioning Machine (RPM), the 2D Fast-Rotating Clinostat (FRC) or the Rotating Wall Vessel (RWV). Subsequently, we investigated self-organization of 3D aggregates without scaffolds pursuing to enhance the frequency of 3D formation and to enlarge the size of the organ-like aggregates. The density of the monolayer exposed to real or simulated microgravity as well as the composition of the culture media revealed an impact on the results. Genomic and proteomic alterations were induced by simulated microgravity. Under microgravity conditions, adherent cells expressed other genes than cells grown in spheroids. In this MiniReview, the recent improvements in scaffold-free tissue formation are summarized and relationships between phenotypic and molecular appearance are highlighted.

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Language(s): eng - English
 Dates: 2016-02-292016-10
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000387025100004
DOI: 10.1111/bcpt.12561
 Degree: -

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Title: Annual Meeting of the Scandinavian-Physiological-Society
Place of Event: Aarhus, DENMARK
Start-/End Date: 2015-09-18 - 2015-09-20

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Title: Basic & Clinical Pharmacology & Toxicology
  Abbreviation : BCPT
Source Genre: Journal
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Publ. Info: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Pages: - Volume / Issue: 119 Sequence Number: - Start / End Page: 26 - 33 Identifier: ISSN: 1742-7835