非表示:
キーワード:
CYTOPLASMIC POLY(A) POLYMERASE; CAENORHABDITIS-ELEGANS GERMLINE;
MESSENGER-RNA; MITOSIS/MEIOSIS DECISION; POLYADENYLATION COMPLEX;
ACTIVATION; DROSOPHILA; OOGENESIS; TRANSLATION; REGULATORBiochemistry & Molecular Biology; translational regulation; cytoplasmic polyadenylation;
nucleotidyl-transferase; germline development; C. elegans;
要旨:
Cytoplasmic polyadenylation drives the translational activation of specific mRNAs in early metazoan development and is performed by distinct complexes that share the same catalytic poly(A)-polymerase subunit, GLD-2. The activity and specificity of GLD-2 depend on its binding partners. In Caenorhabditis elegans, GLD-2 promotes spermatogenesis when bound to GLD-3 and oogenesis when bound to RNP-8. GLD-3 and RNP-8 antagonize each other and compete for GLD-2 binding. Following up on our previous mechanistic studies of GLD-2-GLD-3, we report here the 2.5 resolution structure and biochemical characterization of a GLD-2-RNP-8core complex. In the structure, RNP-8 embraces the poly(A)-polymerase, docking onto several conserved hydrophobic hotspots present on the GLD-2 surface. RNP-8 stabilizes GLD-2 and indirectly stimulates polyadenylation. RNP-8 has a different amino-acid sequence and structure as compared to GLD-3. Yet, it binds the same surfaces of GLD-2 by forming alternative interactions, rationalizing the remarkable versatility of GLD-2 complexes.
