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  Optical Control of Lipid Rafts with Photoswitchable Ceramides

Frank, J. A., Franquelim, H. G., Schwille, P., & Trauner, D. (2016). Optical Control of Lipid Rafts with Photoswitchable Ceramides. Journal of the American Chemical Society, 138(39), 12981-12986. doi:10.1021/jacs.6b07278.

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externe Referenz:
http://pubs.acs.org/doi/abs/10.1021/jacs.6b07278 (Verlagsversion)
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 Urheber:
Frank, James Allen1, Autor
Franquelim, Henri G.2, Autor           
Schwille, Petra2, Autor           
Trauner, Dirk1, Autor
Affiliations:
1external, ou_persistent22              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

Inhalt

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Schlagwörter: LIQUID-ORDERED DOMAINS; BIOPHYSICAL PROPERTIES; SIMPLE SPHINGOLIPIDS; COMBINED AFM; MEMBRANE; BILAYERS; CHAIN; FCS; PHOTOPHARMACOLOGY; SPHINGOMYELINASEChemistry;
 Zusammenfassung: Ceramide is a pro-apoptotic sphingolipid with unique physical characteristics. Often viewed as a second messenger, its generation can modulate the structure of lipid rafts. We prepared three photoswitchable ceramides, Aces, which contain an azobenzene photoswitch allowing for, optical control over the N-acyl chain. Using combined atomic force and confocal fluorescence microscopy, we demonstrate that the ACes enable reversible switching of lipid domains in raft-mimicking supported lipid bilayers (SLBs). In the trans configuration, the ACes localize into the liquid-ordered (L-o) phase. Photoisomerization to the cis-form triggers a fluidification of the L. domains, as liquid-disordered (L-d) "lakes" are formed within the rafts. Photoisomerization back to the trans-state with blue light stimulates a rigidification inside the L-d phase, as the formation of small L. domains. These changes can be repeated over multiple cycles, enabling a dynamic spatiotemporal control of the lipid raft structure with light.

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Sprache(n): eng - English
 Datum: 2016-09-142016-10-05
 Publikationsstatus: Erschienen
 Seiten: 6
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000384952100042
DOI: 10.1021/jacs.6b07278
 Art des Abschluß: -

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Titel: Journal of the American Chemical Society
  Andere : J. Am. Chem. Soc.
  Kurztitel : JACS
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington, DC : American Chemical Society
Seiten: - Band / Heft: 138 (39) Artikelnummer: - Start- / Endseite: 12981 - 12986 Identifikator: ISSN: 0002-7863
CoNE: https://pure.mpg.de/cone/journals/resource/954925376870