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  Local Ras activation, PTEN pattern, and global actin flow in the chemotactic responses of oversized cells

Lange, M., Prassler, J., Ecke, M., Mueller-Taubenberger, A., & Gerisch, G. (2016). Local Ras activation, PTEN pattern, and global actin flow in the chemotactic responses of oversized cells. Journal of Cell Science, 129(18), 3462-3472. doi:10.1242/jcs.191148.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-415B-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-415C-1
Genre: Journal Article

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http://jcs.biologists.org/content/129/18/3462 (Publisher version)
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 Creators:
Lange, Markus1, Author              
Prassler, Jana1, Author              
Ecke, Mary1, Author              
Mueller-Taubenberger, Annette2, Author
Gerisch, Günther1, Author              
Affiliations:
1Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              
2external, ou_persistent22              

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Free keywords: EUKARYOTIC CHEMOTAXIS; FLUORESCENT PROTEIN; PIP3 WAVES; GRADIENTS; MIGRATION; MODELS; DICTYOSTELIUM; INHIBITION; MECHANISMS; MEMBRANECell Biology; Actin flow; Cell fusion; Cell polarity; Chemotaxis; PTEN; Ras;
 Abstract: Chemotactic responses of eukaryotic cells require a signal processing system that translates an external gradient of attractant into directed motion. To challenge the response system to its limits, we increased the size of Dictyostelium discoideum cells by using electric-pulse-induced fusion. Large cells formed multiple protrusions at different sites along the gradient of chemoattractant, independently turned towards the gradient and competed with each other. Finally, these cells succeeded to re-establish polarity by coordinating front and tail activities. To analyse the responses, we combined two approaches, one aimed at local responses by visualising the dynamics of Ras activation at the front regions of reorientating cells, the other at global changes of polarity by monitoring front-to-tail-directed actin flow. Asymmetric Ras activation in turning protrusions underscores that gradients can be sensed locally and translated into orientation. Different to cells of normal size, the polarity of large cells is not linked to an increasing front-to-tail gradient of the PIP3-phosphatase PTEN. But even in large cells, the front communicates with the tail through an actin flow that might act as carrier of a protrusion inhibitor.

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Language(s): eng - English
 Dates: 2016-09-152016
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000384082900009
DOI: 10.1242/jcs.191148
 Degree: -

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Title: Journal of Cell Science
Source Genre: Journal
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Publ. Info: Cambridge, U.K. : Co. of Biologists
Pages: - Volume / Issue: 129 (18) Sequence Number: - Start / End Page: 3462 - 3472 Identifier: ISSN: 0021-9533
CoNE: https://pure.mpg.de/cone/journals/resource/954925326678