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  Current-Induced Transistor Sensorics with Electrogenic Cells

Fromherz, P. (2016). Current-Induced Transistor Sensorics with Electrogenic Cells. Biosensors, 6(2): 18. doi:10.3390/bios6020018.

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biosensors-06-00018-v2.pdf (Publisher version), 4MB
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Fromherz, Peter1, Author           
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1Fromherz, Peter / Membrane and Neurophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565146              

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Free keywords: FIELD-EFFECT TRANSISTOR; INTERFERENCE-CONTRAST MICROSCOPY; NEURON-SILICON JUNCTION; OXIDIZED SILICON; ADHESION; STIMULATION; INHIBITION; POTENTIALS; CHANNELS; SIGNALSChemistry; transistor; extracellular recording; ion channel; neuron; action potential;
 Abstract: The concepts of transistor recording of electroactive cells are considered, when the response is determined by a current-induced voltage in the electrolyte due to cellular activity. The relationship to traditional transistor recording, with an interface-induced response due to interactions with the open gate oxide, is addressed. For the geometry of a cell-substrate junction, the theory of a planar core-coat conductor is described with a one-compartment approximation. The fast electrical relaxation of the junction and the slow change of ion concentrations are pointed out. On that basis, various recording situations are considered and documented by experiments. For voltage-gated ion channels under voltage clamp, the effects of a changing extracellular ion concentration and the enhancement/depletion of ion conductances in the adherent membrane are addressed. Inhomogeneous ion conductances are crucial for transistor recording of neuronal action potentials. For a propagating action potential, the effects of an axon-substrate junction and the surrounding volume conductor are distinguished. Finally, a receptor-transistor-sensor is described, where the inhomogeneity of a ligand-activated ion conductance is achieved by diffusion of the agonist and inactivation of the conductance. Problems with regard to a development of reliable biosensors are mentioned.

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Language(s): eng - English
 Dates: 2016-01-282016-04-25
 Publication Status: Published online
 Pages: 30
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000381858800008
DOI: 10.3390/bios6020018
 Degree: -

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Title: Biosensors
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Source Genre: Journal
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Publ. Info: Basel : MDPI AG
Pages: - Volume / Issue: 6 (2) Sequence Number: 18 Start / End Page: - Identifier: Other: 2079-6374