English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Functional characterization of the DnaK chaperone system from the archaeon Methanothermobacter thermautotrophicus ΔH

Popp, S., & Reinstein, J. (2009). Functional characterization of the DnaK chaperone system from the archaeon Methanothermobacter thermautotrophicus ΔH. FEBS Letters, 583(3), 573-578. doi:10.1016/j.febslet.2008.12.062.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-1234-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-DEDA-1
Genre: Journal Article

Files

show Files
hide Files
:
FEBSLett_583_2009_573.pdf (Any fulltext), 373KB
 
File Permalink:
-
Name:
FEBSLett_583_2009_573.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
FEBSLett_583_2009_573_Suppl.pdf (Supplementary material), 2MB
 
File Permalink:
-
Name:
FEBSLett_583_2009_573_Suppl.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Description:
-

Creators

show
hide
 Creators:
Popp, Simone1, Author              
Reinstein, Jochen1, Author              
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Content

show
hide
Free keywords: DnaK; Chaperone; Archaea; ATPase activity; Luciferase refolding; Methanothermobacter thermautotrophicus
 Abstract: We characterized the biochemical and functional properties of the DnaK system from the archaeon Methanothermobacter thermautotrophicus ΔH. In contrast to the eubacterial chaperone components the archaeal Hsp70 system shows thermal transitions only slightly above the optimal environmental temperature (65 °C). Nevertheless, it prevents aggregation of luciferase in the physiological temperature range of the organism, but is also fully functional at 30 °C in luciferase refolding. Additionally, GrpEM.th. and DnaJM.th. substitute their eubacterial counterparts whereas DnaKM.th. is only functional with its native cochaperones which could be attributed to a functional specialization of the eubacterial chaperones during evolution.

Details

show
hide
Language(s): eng - English
 Dates: 2008-12-192008-10-302008-12-302009-01-202009-02-04
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 583 (3) Sequence Number: - Start / End Page: 573 - 578 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501