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  Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors

Obenhaus, H. A., Rozov, A., Bertocchi, I., Tang, W., Kirsch, J., Betz, H., et al. (2016). Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors. Frontiers in Molecular Neuroscience, 9: 75, pp. 1-17. doi:10.3389/fnmol.2016.00075.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-150E-0 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-3702-B
Genre: Journal Article

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 Creators:
Obenhaus, Horst A.1, Author           
Rozov, Andrei, Author
Bertocchi, Ilaria1, Author           
Tang, Wannan1, Author           
Kirsch, Joachim, Author
Betz, Heinrich2, Author           
Sprengel, Rolf1, 2, Author           
Affiliations:
1Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497741              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: glycine receptor; ivermectin; neuronal silencing; odor discrimination; rAAV
 Abstract: The causal interrogation of neuronal networks involved in specific behaviors requires the spatially and temporally controlled modulation of neuronal activity. For long-term manipulation of neuronal activity, chemogenetic tools provide a reasonable alternative to short-term optogenetic approaches. Here we show that virus mediated gene transfer of the ivermectin (IVM) activated glycine receptor mutant GlyRα1 (AG) can be used for the selective and reversible silencing of specific neuronal networks in mice. In the striatum, dorsal hippocampus, and olfactory bulb, GlyRα1 (AG) promoted IVM dependent effects in representative behavioral assays. Moreover, GlyRα1 (AG) mediated silencing had a strong and reversible impact on neuronal ensemble activity and c-Fos activation in the olfactory bulb. Together our results demonstrate that long-term, reversible and re-inducible neuronal silencing via GlyRα1 (AG) is a promising tool for the interrogation of network mechanisms underlying the control of behavior and memory formation.

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Language(s): eng - English
 Dates: 2016-07-112016-08-102016-08-30
 Publication Status: Published online
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3389/fnmol.2016.00075
URI: https://www.ncbi.nlm.nih.gov/pubmed/27625595
URI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004486/
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Title: Frontiers in Molecular Neuroscience
Source Genre: Journal
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Publ. Info: Lausanne, Switzerland : Frontiers Research Foundation
Pages: - Volume / Issue: 9 Sequence Number: 75 Start / End Page: 1 - 17 Identifier: ISSN: 1662-5099
CoNE: https://pure.mpg.de/cone/journals/resource/1662-5099