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  Visualization of lipids and proteins at high spatial and temporal resolution via interferometric scattering (iSCAT) microscopy

Spindler, S., Ehrig, J., Koenig, K., Nowak, T., Piliarik, M., Stein, H. E., et al. (2016). Visualization of lipids and proteins at high spatial and temporal resolution via interferometric scattering (iSCAT) microscopy. Journal of Physics D: Applied Physics, 49: 349601. doi:10.1088/0022-3727/49/34/349601.

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 Creators:
Spindler, Susann1, Author              
Ehrig, Jens1, Author              
Koenig, Katharina2, Author
Nowak, Tristan2, Author
Piliarik, Marek1, Author              
Stein, Hannah E.2, Author
Taylor, Richard W.2, Author
Garanger, Elisabeth2, Author
Lecommandoux, Sebastien2, Author
Alves, Isabel D.2, Author
Sandoghdar, Vahid1, Author              
Affiliations:
1Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society, ou_2364722              
2external, ou_persistent22              

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Free keywords: Physics;
 Abstract: Microscopy based on the interferometric detection of light scattered from nanoparticles (iSCAT) was introduced in our laboratory more than a decade ago. In this work, we present various capabilities of iSCAT for biological studies by discussing a selection of our recent results. In particular, we show tracking of lipid molecules in supported lipid bilayers (SLBs), tracking of gold nanoparticles with diameters as small as 5 nm and at frame rates close to 1 MHz, 3D tracking of Tat peptide-coated nanoparticles on giant unilamellar vesicles (GUVs), imaging the formation of lipid bilayers, sensing single unlabelled proteins and tracking their motion under electric fields, as well as challenges of studying live cell membranes. These studies set the ground for future quantitative research on dynamic biophysical processes at the nanometer scale.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published online
 Pages: 1
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Journal of Physics D: Applied Physics
  Abbreviation : J. Phys. D: Appl. Phys.
Source Genre: Journal
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Publ. Info: Bristol : IOP Publishing
Pages: - Volume / Issue: 49 Sequence Number: 349601 Start / End Page: - Identifier: ISSN: 0022-3727
CoNE: https://pure.mpg.de/cone/journals/resource/0022-3727