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  Leukocyte responses to immobilized patterns of CXCL8

Girrbach, M., Rink, I., Ladnorg, T., Azucena, C., Heißler, S., Haraszti, T., et al. (2016). Leukocyte responses to immobilized patterns of CXCL8. Colloids and Surfaces B: Biointerfaces, 142, 385-391. doi:10.1016/j.colsurfb.2016.03.004.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-7DCC-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-637F-A
Genre: Journal Article

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ColloidsSurfBBiointerfaces_142_2016_385.pdf (Any fulltext), 873KB
 
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 Creators:
Girrbach, Maria, Author
Rink, Ina, Author
Ladnorg, Tatjana, Author
Azucena, Carlos, Author
Heißler, Stefan, Author
Haraszti, Tamas1, 2, Author              
Schepers, Ute, Author
Schmitz, Katja, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: Biofunctional surfaces; Chemokines; Microcontact printing; Protein patterns
 Abstract: The attachment of neutrophils to the endothelial surface and their migration towards the site of inflammation following chemokine gradients play an essential role in the innate immune response. Chemokines adhere to glycosaminoglycans on the endothelial surface to be detected by leukocytes and trigger their movement along surface- bound gradients in a process called haptotaxis. In assays to systematically study the response of leukocytes to surface-bound compounds both the spatial arrangement of the compound as well as the mode of immobilization need to be controlled. In this study microcontact printing was employed to create patterns of hydrophobic or functionalized thiols on gold-coated glass slides and CXCL8 was immobilized on the thiol coated areas using three different strategies. Human neutrophils adhered to the CXCL8-coated lines but not to the PEG-coated background. We could show that more cells adhered to CXCL8 adsorbed to hydrophobic octadecanethiol than on CXCL8 covalently bound to amino undecanethiol or CXCL8 specifically bound to immobilized heparin on aminothiol. Likewise general cell activity such as lamellipodia formation and random migration were most pronounced for CXCL8 adsorbed on a hydrophobic surface which may be attributed to the larger amounts of protein immobilized on this type of surface.

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Language(s): eng - English
 Dates: 2016-02-022015-12-122016-03-022016-06-01
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Colloids and Surfaces B: Biointerfaces
  Abbreviation : Colloids Surf., B
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 142 Sequence Number: - Start / End Page: 385 - 391 Identifier: ISSN: 0927-7765
CoNE: https://pure.mpg.de/cone/journals/resource/954926242488