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  Controlled immobilization strategies to probe short hyaluronan-protein interactions

Baykal Minsky, B., Antoni, C., & Böhm, H. (2016). Controlled immobilization strategies to probe short hyaluronan-protein interactions. Scientific Reports, 6: 21608, pp. 1-8. doi:10.1038/srep21608.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-154B-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-5E98-2
Genre: Journal Article

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 Creators:
Baykal Minsky, Burcu1, 2, Author              
Antoni, Christiane1, 2, Author              
Böhm, Heike1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: Abt. Spatz
 Abstract: Well-controlled grafting of small hyaluronan oligosaccharides (sHA) enables novel approaches to investigate biological processes such as angiogenesis, immune reactions and cancer metastasis. We develop two strategies for covalent attachment of sHA, a fast high-density adsorption and a two-layer system that allows tuning the density and mode of immobilization. We monitored the sHA adlayer formation and subsequent macromolecular interactions by label-free quartz crystal microbalance with dissipation (QCM-D). The modified surfaces are inert to unspecific protein adsorption, and yet retain the specific binding capacity of sHA. Thus they are an ideal tool to study the interactions of hyaluronan-binding proteins and short hyaluronan molecules as demonstrated by the specific recognition of LYVE-1 and aggrecan. Both hyaladherins recognize sHA and the binding is independent to the presence of the reducing end.

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Language(s): eng - English
 Dates: 2015-09-092016-01-272016-02-17
 Publication Status: Published online
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/srep21608
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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 6 Sequence Number: 21608 Start / End Page: 1 - 8 Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322