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  Photocleavable linker for the patterning of bioactive molecules

Wegner, S. V., Sentürk, O. I., & Spatz, J. P. (2015). Photocleavable linker for the patterning of bioactive molecules. Scientific Reports, 5: 18309, pp. 1-7. doi:10.1038/srep18309.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-AD00-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-79B2-5
Genre: Journal Article

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ScientificRep_5_2015_18309.pdf (Any fulltext), 841KB
 
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 Creators:
Wegner, Seraphine V.1, 2, Author              
Sentürk, Oya I., Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Herein, we report the use of a versatile photocleavable nitrobenzyl linker to micropattern a wide variety of bioactive molecules and photorelease them on demand. On one end, the linker has an NHS group that can be coupled with any amine, such as peptides, proteins or amine-linkers, and on the other end an alkyne for convenient attachment to materials with an azide functional group. This linker was conjugated with NTA-amine or the cell adhesion peptide cRGD to enable straightforward patterning of His6-tagged proteins or cells, respectively, on PEGylated glass surfaces. This approach provides a practical way to control the presentation of a wide variety of bioactive molecules with high spatial and temporal resolution. The extent of photocleavage can also be controlled to tune the biomolecule density and degree of cell attachment to the surface.

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Language(s): eng - English
 Dates: 2015-10-022015-11-162015-12-16
 Publication Status: Published online
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 5 Sequence Number: 18309 Start / End Page: 1 - 7 Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322