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  Nanopore diameters tune strain in extruded fibronectin fibers

Raoufi, M., Das, T., Schoen, I., Vogel, V., Brüggemann, D., & Spatz, J. P. (2015). Nanopore diameters tune strain in extruded fibronectin fibers. Nano Letters, 15(10), 6357-6364. doi:10.1021/acs.nanolett.5b01356.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-DAE8-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-7D6B-7
Genre: Journal Article

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 Creators:
Raoufi, Mohammad1, 2, Author              
Das, Tamal1, 2, Author              
Schoen, Ingmar, Author
Vogel, Viola, Author
Brüggemann, Dorothea1, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: conformational changes; protein nanofiber, AAO template; FRET; extrusion
 Abstract: Fibronectin is present in the extracellular matrix and can be assembled into nanofibers in vivo by undergoing conformational changes. Here, we present a novel approach to prepare fibronectin nanofibers under physiological conditions using an extrusion approach through nanoporous aluminum oxide membranes. This one-step process can prepare nanofiber bundles up to a millimeter in length and with uniform fiber diameters in the nanometer range. Most importantly, by using different pore diameters and protein concentrations in the extrusion process, we could induce varying lasting structural changes in the fibers, which were monitored by Förster resonance energy transfer and should impose different physiological functions.

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Language(s): eng - English
 Dates: 2015-09-112015-04-082015-09-112015-09-112015-10-14
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 15 (10) Sequence Number: - Start / End Page: 6357 - 6364 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403