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  Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin

Maier, T., & Haraszti, T. (2015). Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin. PLoS One, 10(8): e0136432, pp. 1-17. doi:10.1371/journal.pone.0136432.

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Maier, Timo1, 2, Author           
Haraszti, Tamas1, 2, Author           
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1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Filamentous actin is one of the most important cytoskeletal elements. Not only is it responsible for the elastic properties of many cell types, but it also plays a vital role in cellular adhesion and motility. Understanding the bundling kinetics of actin filaments is important in the formation of various cytoskeletal structures, such as filopodia and stress fibers. Utilizing a unique pillar-structured microfluidic device, we investigated the time dependence of bundling kinetics of pillar supported free-standing actin filaments. Microparticles attached to the filaments allowed the measurement of thermal motion, and we found that bundling takes place at lower concentrations than previously found in 3-dimensional actin gels, i.e. actin filaments formed bundles in the presence of 5-12 mM of magnesium chloride in a time-dependent manner. The filaments also displayed long term stability for up to hours after removing the magnesium ions from the buffer, which suggests that there is an extensive hysteresis between cation induced crosslinking and decrosslinking.

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Language(s): eng - English
 Dates: 2015-05-122015-08-042015-08-312015-08-31
 Publication Status: Published in print
 Pages: 17
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 Rev. Type: Peer
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 10 (8) Sequence Number: e0136432 Start / End Page: 1 - 17 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850