English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Key factors for stable retention of fluorophores and labeled biomolecules in droplet-based microfluidics

Janiesch, J.-W., Weiss, M., Kannenberg, G., Hannabuss, J., Surrey, T., Platzman, I., et al. (2015). Key factors for stable retention of fluorophores and labeled biomolecules in droplet-based microfluidics. Analytical Chemistry, 87(4), 2063-2067. doi:10.1021/ac504736e.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-0A29-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-7DDF-5
Genre: Journal Article

Files

show Files
hide Files
:
AnalytChem_87_2015_2063.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
AnalytChem_87_2015_2063.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, Heidelberg; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Locator:
http://pubs.acs.org/doi/suppl/10.1021/ac504736e (Supplementary material)
Description:
-
Locator:
https://dx.doi.org/10.1021/ac504736e (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Janiesch, Jan-Willi1, 2, Author              
Weiss, Marian1, Author              
Kannenberg, Gerri1, 2, Author              
Hannabuss, Jonathon, Author
Surrey, Thomas, Author
Platzman, Ilia1, 2, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Water-in-oil emulsion droplets created in droplet-based microfluidic devices have been tested and used recently as well-defined picoliter-sized 3D compartments for various biochemical and biomedical applications. In many of these applications, fluorescence measurements are applied to reveal the protein content, spatial distribution, and dynamics in the droplets. However, emulsion droplets do not always provide entirely sealed compartments, and partitioning of dyes or labeled molecules to the oil phase is frequently observed. Therefore, stable molecular retention in the droplets represents a challenge, and many physical and chemical key factors of microfluidic system components have to be considered. In this study, we investigated the retention of 12 commonly used water-soluble dyes in droplets having six different aqueous phase conditions. We demonstrate that the physicochemical properties of the dyes have a major influence on the retention level. In particular, hydrophilicity has a strong influence on retention, with highly hydrophilic dyes (LogD < -7) showing stable, buffer/medium independent retention. In the case of less hydrophilic dyes, we showed that retention can be improved by adjusting the surfactants physical properties, such as geometry, length, and concentration. Furthermore, we analyzed the retention stability of labeled biomolecules such as antibodies, streptavidin, and tubulin proteins and showed that stable retention can be strongly dependent on dye and surfactants selection.

Details

show
hide
Language(s): eng - English
 Dates: 2014-12-192015-01-212015-01-262015-01-21
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Analytical Chemistry
  Abbreviation : Anal. Chem.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 87 (4) Sequence Number: - Start / End Page: 2063 - 2067 Identifier: Other: 0003-2700
CoNE: /journals/resource/111032812862552