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  Surface-mediated priming during in vitro generation of monocyte-derived dendritic cells

Sauter, A., Mc Duffie, Y., Böhm, H., Martinez, A., Spatz, J. P., & Appel, S. (2015). Surface-mediated priming during in vitro generation of monocyte-derived dendritic cells. Scandinavian Journal of Immunology, 81(1), 56-65. doi:10.1111/sji.12246.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-C23C-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-5E89-4
Genre: Journal Article

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ScandJImmunol_81_2015_56.pdf (Any fulltext), 689KB
 
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 Creators:
Sauter, Alexander, Author
Mc Duffie, Yvonne, Author              
Böhm, Heike1, 2, Author              
Martinez, Aurora, Author
Spatz, Joachim P.1, 2, Author              
Appel, Silke, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Ex vivo-generated human dendritic cells (DC) are most commonly generated from monocytes using standard cell culture dishes. To elucidate the effect of the plastic surface during the differentiation process, we compared a standard adhesive plastic dish with four different mainly non-adherent surfaces. Untouched monocytes were cultured for 3 days in the presence of IL-4 and GM-CSF. Time-lapse videos were recorded, and the phenotype of the cells was analysed by flow cytometry. The cytokine profiles were analysed using a 25-plex cytokine assay. The use of non-adherent surfaces led to a significant reduction in expression of CD14 and CD38, and a significant increase in expression of CD86 compared to standard culture dishes. Expression levels of DC-SIGN and PD-L2 were reduced significantly on cells cultured on non-adherent surfaces. The cytokine production was independent on the surface used. The surface-mediated priming should therefore be considered when aiming to induce specific immune responses. This is especially important with regard to DC-based immunotherapy, where an adjustment of the surface during the DC generation process might have highly beneficial effects.

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Language(s): eng - English
 Dates: 2014-06-162014-10-212015-01-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Scandinavian Journal of Immunology
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 81 (1) Sequence Number: - Start / End Page: 56 - 65 Identifier: ISSN: 0300-9475
CoNE: https://pure.mpg.de/cone/journals/resource/954927647729